RegulonDB RegulonDB 11.1: Gene Form
   

kbl gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

kbl tdh htrL Lrp Lrp terminator anti-anti-terminator anti-terminator kblp kblp

Gene      
Name: kbl    Texpresso search in the literature
Synonym(s): ECK3607, EG10512, b3617
Genome position(nucleotides): 3791355 <-- 3792551
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.22
Reference(s): [1] Aronson BD., et al., 1988
[2] Rex JH., et al., 1991
External database links:  
ASAP:
 ABE-0011832
CGSC:
 18205
ECHOBASE:
 EB0507
ECOLIHUB:
 kbl
OU-MICROARRAY:
 b3617
STRING:
 511145.b3617
COLOMBOS: kbl


Product      
Name: 2-amino-3-ketobutyrate CoA ligase
Synonym(s): Kbl
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 43.117
Isoelectric point: 5.83
Motif(s):
 
Type Positions Sequence Comment
43 -> 43 H UniProt: In Ref. 1; CAA29883..
111 -> 112 CF UniProt: Pyridoxal phosphate binding; Sequence Annotation Type: region of interest.
171 -> 171 A UniProt: In Ref. 1; CAA29883..
183 -> 183 V UniProt: In Ref. 1; CAA29883..
210 -> 213 DDSH UniProt: Pyridoxal phosphate binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.30 - threonine catabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0016746 - acyltransferase activity
GO:0030170 - pyridoxal phosphate binding
GO:0008890 - glycine C-acetyltransferase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0009058 - biosynthetic process
GO:0006567 - threonine catabolic process
GO:0019518 - L-threonine catabolic process to glycine
External database links:  
ALPHAFOLD:
 P0AB77
DIP:
 DIP-48030N
ECOCYC:
 AKBLIG-MONOMER
ECOLIWIKI:
 b3617
INTERPRO:
 IPR015424
INTERPRO:
 IPR015421
INTERPRO:
 IPR015422
INTERPRO:
 IPR011282
INTERPRO:
 IPR001917
INTERPRO:
 IPR004839
MODBASE:
 P0AB77
PDB:
 1FC4
PFAM:
 PF00155
PRIDE:
 P0AB77
PRODB:
 PRO_000023046
PROSITE:
 PS00599
REFSEQ:
 NP_418074
SMR:
 P0AB77
UNIPROT:
 P0AB77


Operon      
Name: kbl-tdh         
Operon arrangement:
Transcription unit        Promoter
kbl-tdh


Transcriptional Regulation      
Display Regulation             
Activated by: Lrp


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Aronson BD., Ravnikar PD., Somerville RL., 1988, Nucleotide sequence of the 2-amino-3-ketobutyrate coenzyme A ligase (kbl) gene of E. coli., Nucleic Acids Res 16(8):3586

 [2] Rex JH., Aronson BD., Somerville RL., 1991, The tdh and serA operons of Escherichia coli: mutational analysis of the regulatory elements of leucine-responsive genes., J Bacteriol 173(19):5944-53

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