RegulonDB

Gene
Name:	kdpA
Synonym(s):	ECK0686, EG10513, b0698, kac
Genome position(nucleotides):	727059 <-- 728732 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.5
External database links:

ASAP:	ABE-0002381
CGSC:	588
ECHOBASE:	EB0508
ECOLIHUB:	kdpA
OU-MICROARRAY:	b0698
STRING:	511145.b0698
COLOMBOS:	kdpA

Product

Name:

K⁺ transporting P-type ATPase subunit KdpA

Synonym(s):

Kac, KdpA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

59.189

Isoelectric point:

7.468

Motif(s):

Type	Positions	Sequence
	11 -> 556	TFLLVLMVLARPLGSGLARLINDIPLPGTTGVERVLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLALNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSMSTLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGPVASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVMGDRRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGTDSSINMEGKESRFGVLVSSLFAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGPHGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQAASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYL
	231 -> 231	N
	5 -> 25	GFLLIATFLLVLMVLARPLGS
	170 -> 190	LLRITLWVLVPVALLIALFFI
	283 -> 303	LLWAMSVIFVICVGVVMWAEV

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.3 - The P-type ATPase (P-ATPase) Superfamily
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0031004 - potassium ion-transporting ATPase complex GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0005515 - protein binding GO:0008556 - potassium transmembrane transporter activity, phosphorylative mechanism GO:0030955 - potassium ion binding
	biological_process	GO:0006811 - ion transport GO:0006813 - potassium ion transport GO:0071805 - potassium ion transmembrane transport GO:0098655 - cation transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Bertrand J., et al., 2004
[2] Dorus S., et al., 2001
[3] Sardesai AA., et al., 2001
[4] Schrader M., et al., 2000

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PANTHER:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

kdpFABC

Operon arrangement:

Transcription unit	Promoter
kdpFABC	kdpFp

Transcriptional Regulation
	Display Regulation
Activated by:	KdpE

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	ybfAp7	729037	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 4.6. P-value: 2.232e-5 Read more >	[ICWHO]	[5]
promoter	TSS_828 (cluster)	729071	forward	For this promoter, there is a cluster of 3 transcription start sites,in the interval 729071 to 729073, where the highest frequency is at position 728295. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[6]
promoter	ybfAp8	729082	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 6.116. P-value: 1.327e-5 Read more >	[ICWHO], [RS-EPT-CBR]	[5], [6]
promoter	TSS_830	729088	forward	nd	[RS-EPT-CBR]	[6]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Bertrand J., Altendorf K., Bramkamp M., 2004, Amino acid substitutions in putative selectivity filter regions III and IV in KdpA alter ion selectivity of the KdpFABC complex from Escherichia coli., J Bacteriol 186(16):5519-22
[2] Dorus S., Mimura H., Epstein W., 2001, Substrate-binding clusters of the K+-transporting Kdp ATPase of Escherichia coli investigated by amber suppression scanning mutagenesis., J Biol Chem 276(13):9590-8
[3] Sardesai AA., Gowrishankar J., 2001, Improvement in K+-limited growth rate associated with expression of the N-terminal fragment of one subunit (KdpA) of the multisubunit Kdp transporter in Escherichia coli., J Bacteriol 183(11):3515-20
[4] Schrader M., Fendler K., Bamberg E., Gassel M., Epstein W., Altendorf K., Drose S., 2000, Replacement of glycine 232 by aspartic acid in the KdpA subunit broadens the ion specificity of the K(+)-translocating KdpFABC complex., Biophys J 79(2):802-13
[5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.