RegulonDB RegulonDB 10.10: Gene Form
   

lacY gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

lacZ lacY lacA LacI

Gene      
Name: lacY    Texpresso search in the literature
Synonym(s): ECK0340, EG10526, b0343
Genome position(nucleotides): 361926 <-- 363179 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 46.33
External database links:  
ASAP:
 ABE-0001181
CGSC:
 577
ECHOBASE:
 EB0521
ECOLIHUB:
 lacY
OU-MICROARRAY:
 b0343
STRING:
 511145.b0343
COLOMBOS: lacY


Product      
Name: lactose permease
Synonym(s): LacY, galactoside:proton symporter, lac permease, lactose/melibiose:H+ symporter
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 46.503
Isoelectric point: 8.843
Motif(s):
 
Type Positions Sequence
2 -> 412 YYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQ
75 -> 100 YLLWIITGMLVMFAPFFIFIFGPLLQ
96 -> 96 G
141 -> 163 GRARMFGCVGWALCASIVGIMFT
154 -> 154 C

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0015293 - symporter activity
GO:0005351 - carbohydrate:proton symporter activity
GO:0022857 - transmembrane transporter activity
GO:0015528 - lactose:proton symporter activity
GO:0030395 - lactose binding
biological_process GO:0008643 - carbohydrate transport
GO:0055085 - transmembrane transport
GO:0015767 - lactose transport
GO:0034219 - carbohydrate transmembrane transport
GO:0071702 - organic substance transport
GO:1902600 - proton transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Andersson M., et al., 2012
[2] Carrasco N., et al., 1984
[3] Carrasco N., et al., 1982
[4] Chaptal V., et al., 2011
[5] Culham DE., et al., 2008
[6] Ermolova N., et al., 2006
[7] Frillingos S., et al., 1997
[8] Garcia-Celma JJ., et al., 2009
[9] Grytsyk N., et al., 2019
[10] Grytsyk N., et al., 2017
[11] Hariharan P., et al., 2016
[12] Harris NJ., et al., 2020
[13] Herzlinger D., et al., 1984
[14] Jiang X., et al., 2011
[15] Jiang X., et al., 2016
[16] Kaback HR. 1997
[17] Kimanius D., et al., 2018
[18] Kwaw I., et al., 2001
[19] Madej MG., et al., 2012
[20] Nie Y., et al., 2009
[21] Pendse PY., et al., 2010
[22] Sahin-Toth M., et al., 1994
[23] Sahin-Toth M., et al., 1993
[24] Sahin-Toth M., et al., 1994
[25] Sandermann H. 1977
[26] Seckler R., et al., 1984
[27] Seckler R., et al., 1983
[28] Smirnova I., et al., 2014
[29] Smirnova I., et al., 2015
[30] Smirnova I., et al., 2018
[31] Smirnova I., et al., 2011
[32] Smirnova I., et al., 2013
[33] Stelzl LS., et al., 2014
[34] Vazquez-Ibar JL., et al., 2004
[35] Vitrac H., et al., 2015
External database links:  
DIP:
 DIP-10080N
ECOCYC:
 LACY-MONOMER
ECOLIWIKI:
 b0343
INTERPRO:
 IPR036259
INTERPRO:
 IPR000576
INTERPRO:
 IPR018457
INTERPRO:
 IPR020846
MODBASE:
 P02920
PDB:
 6C9W
PDB:
 5GXB
PDB:
 4ZYR
PDB:
 2Y5Y
PDB:
 2V8N
PDB:
 2CFQ
PDB:
 2CFP
PDB:
 1PV7
PDB:
 1PV6
PDB:
 1M2U
PFAM:
 PF01306
PRIDE:
 P02920
PRINTS:
 PR00174
PRODB:
 PRO_000023071
PROSITE:
 PS00896
PROSITE:
 PS50850
PROSITE:
 PS00897
REFSEQ:
 NP_414877
SMR:
 P02920
UNIPROT:
 P02920


Operon      
Name: lacZYA         
Operon arrangement:
Transcription unit        Promoter
lacZYA
lacZYA
lacZYA
lacZYA
lacYA


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: CRP, LacI, MarA, H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Andersson M., Bondar AN., Freites JA., Tobias DJ., Kaback HR., White SH., 2012, Proton-coupled dynamics in lactose permease., Structure 20(11):1893-904

 [2] Carrasco N., Herzlinger D., Mitchell R., DeChiara S., Danho W., Gabriel TF., Kaback HR., 1984, Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes., Proc Natl Acad Sci U S A 81(15):4672-6

 [3] Carrasco N., Tahara SM., Patel L., Goldkorn T., Kaback HR., 1982, Preparation, characterization, and properties of monoclonal antibodies against the lac carrier protein from Escherichia coli., Proc Natl Acad Sci U S A 79(22):6894-8

 [4] Chaptal V., Kwon S., Sawaya MR., Guan L., Kaback HR., Abramson J., 2011, Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition., Proc Natl Acad Sci U S A 108(23):9361-6

 [5] Culham DE., Romantsov T., Wood JM., 2008, Roles of K+, H+, H2O, and DeltaPsi in solute transport mediated by major facilitator superfamily members ProP and LacY., Biochemistry 47(31):8176-85

 [6] Ermolova N., Madhvani RV., Kaback HR., 2006, Site-directed alkylation of cysteine replacements in the lactose permease of Escherichia coli: helices I, III, VI, and XI., Biochemistry 45(13):4182-9

 [7] Frillingos S., Sun J., Gonzalez A., Kaback HR., 1997, Cysteine-scanning mutagenesis of helix II and flanking hydrophilic domains in the lactose permease of Escherichia coli., Biochemistry 36(1):269-73

 [8] Garcia-Celma JJ., Smirnova IN., Kaback HR., Fendler K., 2009, Electrophysiological characterization of LacY., Proc Natl Acad Sci U S A 106(18):7373-8

 [9] Grytsyk N., Santos Seica AF., Sugihara J., Kaback HR., Hellwig P., 2019, Arg302 governs the pKa of Glu325 in LacY., Proc Natl Acad Sci U S A 116(11):4934-4939

 [10] Grytsyk N., Sugihara J., Kaback HR., Hellwig P., 2017, pKa of Glu325 in LacY., Proc Natl Acad Sci U S A 114(7):1530-1535

 [11] Hariharan P., Andersson M., Jiang X., Pardon E., Steyaert J., Kaback HR., Guan L., 2016, Thermodynamics of Nanobody Binding to Lactose Permease., Biochemistry 55(42):5917-5926

 [12] Harris NJ., Pellowe GA., Booth PJ., 2020, Cell-free expression tools to study co-translational folding of alpha helical membrane transporters., Sci Rep 10(1):9125

 [13] Herzlinger D., Viitanen P., Carrasco N., Kaback HR., 1984, Monoclonal antibodies against the lac carrier protein from Escherichia coli. 2. Binding studies with membrane vesicles and proteoliposomes reconstituted with purified lac carrier protein., Biochemistry 23(16):3688-93

 [14] Jiang X., Nie Y., Kaback HR., 2011, Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport., Biochemistry 50(10):1634-40

 [15] Jiang X., Smirnova I., Kasho V., Wu J., Hirata K., Ke M., Pardon E., Steyaert J., Yan N., Kaback HR., 2016, Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation., Proc Natl Acad Sci U S A 113(44):12420-12425

 [16] Kaback HR., 1997, A molecular mechanism for energy coupling in a membrane transport protein, the lactose permease of Escherichia coli., Proc Natl Acad Sci U S A 94(11):5539-43

 [17] Kimanius D., Lindahl E., Andersson M., 2018, Uptake dynamics in the Lactose permease (LacY) membrane protein transporter., Sci Rep 8(1):14324

 [18] Kwaw I., Zen KC., Hu Y., Kaback HR., 2001, Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helices IV and V that contain the major determinants for substrate binding., Biochemistry 40(35):10491-9

 [19] Madej MG., Soro SN., Kaback HR., 2012, Apo-intermediate in the transport cycle of lactose permease (LacY)., Proc Natl Acad Sci U S A 109(44):E2970-8

 [20] Nie Y., Zhou Y., Kaback HR., 2009, Clogging the periplasmic pathway in LacY., Biochemistry 48(4):738-43

 [21] Pendse PY., Brooks BR., Klauda JB., 2010, Probing the periplasmic-open state of lactose permease in response to sugar binding and proton translocation., J Mol Biol 404(3):506-21

 [22] Sahin-Toth M., Frillingos S., Bibi E., Gonzalez A., Kaback HR., 1994, The role of transmembrane domain III in the lactose permease of Escherichia coli., Protein Sci 3(12):2302-10

 [23] Sahin-Toth M., Kaback HR., 1993, Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli., Protein Sci 2(6):1024-33

 [24] Sahin-Toth M., Persson B., Schwieger J., Cohan P., Kaback HR., 1994, Cysteine scanning mutagenesis of the N-terminal 32 amino acid residues in the lactose permease of Escherichia coli., Protein Sci 3(2):240-7

 [25] Sandermann H., 1977, beta-D-Galactoside transport in Escherichia coli: substrate recognition., Eur J Biochem 80(2):507-15

 [26] Seckler R., Wright JK., 1984, Sidedness of native membrane vesicles of Escherichia coli and orientation of the reconstituted lactose: H+ carrier., Eur J Biochem 142(2):269-79

 [27] Seckler R., Wright JK., Overath P., 1983, Peptide-specific antibody locates the COOH terminus of the lactose carrier of Escherichia coli on the cytoplasmic side of the plasma membrane., J Biol Chem 258(18):10817-20

 [28] Smirnova I., Kasho V., Jiang X., Pardon E., Steyaert J., Kaback HR., 2014, Outward-facing conformers of LacY stabilized by nanobodies., Proc Natl Acad Sci U S A 111(52):18548-53

 [29] Smirnova I., Kasho V., Jiang X., Pardon E., Steyaert J., Kaback HR., 2015, Transient conformers of LacY are trapped by nanobodies., Proc Natl Acad Sci U S A 112(45):13839-44

 [30] Smirnova I., Kasho V., Kaback HR., 2018, Engineered occluded apo-intermediate of LacY., Proc Natl Acad Sci U S A 115(50):12716-12721

 [31] Smirnova I., Kasho V., Sugihara J., Kaback HR., 2011, Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding., Proc Natl Acad Sci U S A 108(37):15147-51

 [32] Smirnova I., Kasho V., Sugihara J., Kaback HR., 2013, Trp replacements for tightly interacting Gly-Gly pairs in LacY stabilize an outward-facing conformation., Proc Natl Acad Sci U S A 110(22):8876-81

 [33] Stelzl LS., Fowler PW., Sansom MS., Beckstein O., 2014, Flexible gates generate occluded intermediates in the transport cycle of LacY., J Mol Biol 426(3):735-51

 [34] Vazquez-Ibar JL., Guan L., Weinglass AB., Verner G., Gordillo R., Kaback HR., 2004, Sugar recognition by the lactose permease of Escherichia coli., J Biol Chem 279(47):49214-21

 [35] Vitrac H., MacLean DM., Jayaraman V., Bogdanov M., Dowhan W., 2015, Dynamic membrane protein topological switching upon changes in phospholipid environment., Proc Natl Acad Sci U S A 112(45):13874-9

RegulonDB