RegulonDB RegulonDB 10.9: Gene Form
   

mdh gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

degS mdh argR ArgR ArgR BtsR BtsR CRP BtsR ArcA argRp1 argRp1 argRp2 argRp2 TSS_3572 TSS_3572 mdhp2 mdhp2 TSS_3571 TSS_3571 mdhp1 mdhp1 TSS_3570 (cluster) TSS_3570 (cluster) TSS_3569 TSS_3569 TSS_3568 (cluster) TSS_3568 (cluster)

Gene      
Name: mdh    Texpresso search in the literature
Synonym(s): ECK3225, EG10576, b3236
Genome position(nucleotides): 3383330 <-- 3384268 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.29
External database links:  
ASAP:
ABE-0010613
CGSC:
523
ECHOBASE:
EB0571
ECOLIHUB:
mdh
OU-MICROARRAY:
b3236
STRING:
511145.b3236
COLOMBOS: mdh


Product      
Name: malate dehydrogenase
Synonym(s): Mdh
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 32.337
Isoelectric point: 5.461
Motif(s):
 
Type Positions Sequence
70 -> 70 A
289 -> 289 Q
37 -> 37 P
116 -> 116 I
106 -> 106 A

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
  1 - metabolism --> 1.7 - central intermediary metabolism
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.21 - glyoxylate degradation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0019898 - extrinsic component of membrane
molecular_function GO:0003824 - catalytic activity
GO:0016491 - oxidoreductase activity
GO:0016616 - oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0016615 - malate dehydrogenase activity
GO:0030060 - L-malate dehydrogenase activity
biological_process GO:0005975 - carbohydrate metabolic process
GO:0019752 - carboxylic acid metabolic process
GO:0006096 - glycolytic process
GO:0006099 - tricarboxylic acid cycle
GO:0006113 - fermentation
GO:0009061 - anaerobic respiration
GO:0006108 - malate metabolic process
GO:0055114 - oxidation-reduction process
External database links:  
DIP:
DIP-35924N
ECOCYC:
MALATE-DEHASE-MONOMER
ECOLIWIKI:
b3236
INTERPRO:
IPR015955
INTERPRO:
IPR010097
INTERPRO:
IPR001557
INTERPRO:
IPR001236
INTERPRO:
IPR022383
INTERPRO:
IPR023958
INTERPRO:
IPR036291
INTERPRO:
IPR001252
MODBASE:
P61889
PANTHER:
PTHR11540:SF22
PDB:
5Z3W
PDB:
5KKA
PDB:
3HHP
PDB:
2PWZ
PDB:
2CMD
PDB:
1IE3
PDB:
1IB6
PDB:
1EMD
PDB:
1CME
PFAM:
PF00056
PFAM:
PF02866
PRIDE:
P61889
PRODB:
PRO_000023174
PROSITE:
PS00068
REFSEQ:
NP_417703
SMR:
P61889
UNIPROT:
P61889


Operon      
Name: mdh         
Operon arrangement:
Transcription unit        Promoter
mdh
mdh


Transcriptional Regulation      
Display Regulation             
Activated by: DpiA, CRP
Repressed by: ArcA, FlhDC, BtsR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3568 (cluster) 3382929 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3569 3384285 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3570 (cluster) 3384294 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3571 3384314 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3572 3384472 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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