RegulonDB RegulonDB 10.9: Gene Form
   

melB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

melB melA yjdF terminator

Gene      
Name: melB    Texpresso search in the literature
Synonym(s): ECK4113, EG10578, b4120, mel-4
Genome position(nucleotides): 4343369 --> 4344790 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
45.85
External database links:  
ASAP:
ABE-0013495
CGSC:
521
ECHOBASE:
EB0573
ECOLIHUB:
melB
OU-MICROARRAY:
b4120
STRING:
511145.b4120
COLOMBOS: melB


Product      
Name: melibiose:H+/Na+/Li+ symporter
Synonym(s): MelB, MelB GPH transporter
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 52.636
Isoelectric point: 8.682
Motif(s):
 
Type Positions Sequence
43 -> 63 LVGTLFLVARIWDAINDPIMG
329 -> 349 IAGILLNVGTALFWVLQVIMV
154 -> 174 LAGFVTAGVTLPFVNYVGGGD
236 -> 256 LLGMALAYNVASNIITGFAIY
2 -> 473 SISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADIVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0015293 - symporter activity
GO:0022857 - transmembrane transporter activity
GO:0015486 - glycoside-pentoside-hexuronide:cation symporter activity
GO:0015487 - melibiose:cation symporter activity
GO:0015592 - methylgalactoside transmembrane transporter activity
GO:0043887 - melibiose:sodium symporter activity
biological_process GO:0008643 - carbohydrate transport
GO:0006814 - sodium ion transport
GO:0055085 - transmembrane transport
GO:0034219 - carbohydrate transmembrane transport
GO:0071702 - organic substance transport
GO:0035725 - sodium ion transmembrane transport
GO:0015765 - methylgalactoside transport
GO:0015769 - melibiose transport
Note(s): Note(s): ...[more].
Reference(s): [1] Abdel-Dayem M., et al., 2003
[2] Ganea C., et al., 2009
[3] Ganea C., et al., 2001
[4] Garcia-Celma JJ., et al., 2008
[5] Granell M., et al., 2010
[6] Leon X., et al., 2005
[7] Meyer-Lipp K., et al., 2004
[8] Meyer-Lipp K., et al., 2006
[9] Pourcher T., et al., 1991
[10] Pourcher T., et al., 1995
[11] Pourcher T., et al., 1993
[12] Purhonen P., et al., 2005
[13] Tsuchiya T., et al., 1985
[14] Yousef MS., et al., 2009
[15] Zani ML., et al., 1994
[16] Zani ML., et al., 1993
External database links:  
DIP:
DIP-10180N
ECOCYC:
MELB-MONOMER
ECOLIWIKI:
b4120
INTERPRO:
IPR036259
INTERPRO:
IPR018043
INTERPRO:
IPR001927
INTERPRO:
IPR039672
PANTHER:
PTHR11328
PFAM:
PF13347
PRIDE:
P02921
PRODB:
PRO_000023198
PROSITE:
PS00872
REFSEQ:
NP_418544
UNIPROT:
P02921


Operon      
Name: melAB         
Operon arrangement:
Transcription unit        Promoter
melAB


Transcriptional Regulation      
Display Regulation             
Activated by: MelR, CRP
Repressed by: MelR


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Abdel-Dayem M., Basquin C., Pourcher T., Cordat E., Leblanc G., 2003, Cytoplasmic loop connecting helices IV and V of the melibiose permease from Escherichia coli is involved in the process of Na+-coupled sugar translocation., J Biol Chem 278(3):1518-24

 [2] Ganea C., Fendler K., 2009, Bacterial transporters: Charge translocation and mechanism., Biochim Biophys Acta 1787(6):706-13

 [3] Ganea C., Pourcher T., Leblanc G., Fendler K., 2001, Evidence for intraprotein charge transfer during the transport activity of the melibiose permease from Escherichia coli., Biochemistry 40(45):13744-52

 [4] Garcia-Celma JJ., Dueck B., Stein M., Schlueter M., Meyer-Lipp K., Leblanc G., Fendler K., 2008, Rapid activation of the melibiose permease MelB immobilized on a solid-supported membrane., Langmuir 24(15):8119-26

 [5] Granell M., Leon X., Leblanc G., Padros E., Lorenz-Fonfria VA., 2010, Structural insights into the activation mechanism of melibiose permease by sodium binding., Proc Natl Acad Sci U S A 107(51):22078-83

 [6] Leon X., Lorenz-Fonfria VA., Lemonnier R., Leblanc G., Padros E., 2005, Substrate-induced conformational changes of melibiose permease from Escherichia coli studied by infrared difference spectroscopy., Biochemistry 44(9):3506-14

 [7] Meyer-Lipp K., Ganea C., Pourcher T., Leblanc G., Fendler K., 2004, Sugar binding induced charge translocation in the melibiose permease from Escherichia coli., Biochemistry 43(39):12606-13

 [8] Meyer-Lipp K., Sery N., Ganea C., Basquin C., Fendler K., Leblanc G., 2006, The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding., J Biol Chem 281(36):25882-92

 [9] Pourcher T., Deckert M., Bassilana M., Leblanc G., 1991, Melibiose permease of Escherichia coli: mutation of aspartic acid 55 in putative helix II abolishes activation of sugar binding by Na+ ions., Biochem Biophys Res Commun 178(3):1176-81

 [10] Pourcher T., Leclercq S., Brandolin G., Leblanc G., 1995, Melibiose permease of Escherichia coli: large scale purification and evidence that H+, Na+, and Li+ sugar symport is catalyzed by a single polypeptide., Biochemistry 34(13):4412-20

 [11] Pourcher T., Zani ML., Leblanc G., 1993, Mutagenesis of acidic residues in putative membrane-spanning segments of the melibiose permease of Escherichia coli. I. Effect on Na(+)-dependent transport and binding properties., J Biol Chem 268(5):3209-15

 [12] Purhonen P., Lundback AK., Lemonnier R., Leblanc G., Hebert H., 2005, Three-dimensional structure of the sugar symporter melibiose permease from cryo-electron microscopy., J Struct Biol 152(1):76-83

 [13] Tsuchiya T., Wilson DM., Wilson TH., 1985, Melibiose-cation cotransport system of Escherichia coli., Ann N Y Acad Sci 456:342-9

 [14] Yousef MS., Guan L., 2009, A 3D structure model of the melibiose permease of Escherichia coli represents a distinctive fold for Na+ symporters., Proc Natl Acad Sci U S A 106(36):15291-6

 [15] Zani ML., Pourcher T., Leblanc G., 1994, Mutation of polar and charged residues in the hydrophobic NH2-terminal domains of the melibiose permease of Escherichia coli., J Biol Chem 269(40):24883-9

 [16] Zani ML., Pourcher T., Leblanc G., 1993, Mutagenesis of acidic residues in putative membrane-spanning segments of the melibiose permease of Escherichia coli. II. Effect on cationic selectivity and coupling properties., J Biol Chem 268(5):3216-21


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