RegulonDB RegulonDB 10.9: Gene Form
   

metF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

metL katG metF OxyR OxyR OxyR FNR FNR MetJ MetJ MetJ MetJ MetJ MetJ MetJ anti-terminator anti-anti-terminator terminator anti-terminator anti-anti-terminator TSS_4650 TSS_4650 TSS_4649 TSS_4649 katGp katGp TSS_4648 TSS_4648 TSS_4647 TSS_4647 TSS_4646 TSS_4646 TSS_4645 (cluster) TSS_4645 (cluster) metFp metFp

Gene      
Name: metF    Texpresso search in the literature
Synonym(s): ECK3933, EG10585, b3941
Genome position(nucleotides): 4132616 --> 4133506 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.85
External database links:  
ASAP:
ABE-0012897
CGSC:
511
ECHOBASE:
EB0580
ECOLIHUB:
metF
MIM:
236250
MIM:
601367
MIM:
601634
MIM:
603174
NCBI-GENE:
948432
OU-MICROARRAY:
b3941
STRING:
511145.b3941
COLOMBOS: metF


Shine dalgarno      
Sequence: agcgattgatGAGGTAaggtATG


Product      
Name: 5,10-methylenetetrahydrofolate reductase
Synonym(s): MetF
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 33.103
Isoelectric point: 6.402
Motif(s):
 
Type Positions Sequence
177 -> 177 A
28 -> 33 EFFPPR
59 -> 60 TY
156 -> 159 HPEA
165 -> 172 DLLNLKRK

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.17 - formyl-THF biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0032991 - protein-containing complex
molecular_function GO:0016491 - oxidoreductase activity
GO:0004489 - methylenetetrahydrofolate reductase (NAD(P)H) activity
GO:0051087 - chaperone binding
GO:0071949 - FAD binding
biological_process GO:0006555 - methionine metabolic process
GO:0006730 - one-carbon metabolic process
GO:0008652 - cellular amino acid biosynthetic process
GO:0009086 - methionine biosynthetic process
GO:0055114 - oxidation-reduction process
GO:0035999 - tetrahydrofolate interconversion
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-6848N
ECOCYC:
METHYLENETHFREDUCT-MONOMER
ECOLIWIKI:
b3941
INTERPRO:
IPR029041
INTERPRO:
IPR004620
INTERPRO:
IPR003171
MODBASE:
P0AEZ1
PDB:
6PEY
PDB:
3FST
PDB:
3FSU
PDB:
1B5T
PDB:
1ZP3
PDB:
1ZP4
PDB:
1ZPT
PDB:
1ZRQ
PDB:
2FMN
PDB:
2FMO
PFAM:
PF02219
PRIDE:
P0AEZ1
PRODB:
PRO_000023212
REFSEQ:
NP_418376
SMR:
P0AEZ1
SWISSMODEL:
P0AEZ1
UNIPROT:
P0AEZ1


Operon      
Name: metF         
Operon arrangement:
Transcription unit        Promoter
metF


Transcriptional Regulation      
Display Regulation             
Repressed by: MetJ


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4645 (cluster) 4133788 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4646 4133795 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4647 4133800 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4648 4133810 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4649 4133980 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4650 4135811 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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