Gene
Name:	mglB
Synonym(s):	ECK2143, EG10593, b2150
Genome position(nucleotides):	2239350 <-- 2240348 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.05
External database links:

ASAP:	ABE-0007108
CGSC:	501
ECHOBASE:	EB0588
ECOLIHUB:	mglB
OU-MICROARRAY:	b2150
STRING:	511145.b2150
COLOMBOS:	mglB

Transcriptional Regulation
	Display Regulation
Activated by:	CRP
Repressed by:	GalS, FlhDC, GalR

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	mglCp5	2237858	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 2.985. P-value: 8.440e-5 Read more >	[ICWHO]	[26]
promoter	mglCp9	2237923	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 4.45. P-value: 2.254e-5 It was suggested that this predicted promoter does not exist, because its activity was not observed during σE induction in high-throughput analysis Lacoux C,2020. Read more >	[ICWHO]	[26]
promoter	mglCp6	2237959	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 2.99. P-value: 8.028e-5 It was suggested that this predicted promoter does not exist, because its activity was not observed during σE induction in high-throughput analysis Lacoux C,2020. Read more >	[ICWHO]	[26]

Reference(s)
[1] Anraku Y., 1968, Transport of sugars and amino acids in bacteria. I. Purification and specificity of the galactose- and leucine-binding proteins., J Biol Chem 243(11):3116-22
[2] Boos W., 1972, Structurally defective galactose-binding protein isolated from a mutant negative in the -methylgalactoside transport system of Escherichia coli., J Biol Chem 247(17):5414-24
[3] Boos W., 1969, The galactose binding protein and its relationship to the beta-methylgalactoside permease from Escherichia coli., Eur J Biochem 10(1):66-73
[4] Boos W., Gordon AS., Hall RE., Price HD., 1972, Transport properties of the galactose-binding protein of Escherichia coli. Substrate-induced conformational change., J Biol Chem 247(3):917-24
[5] D'Auria S., Alfieri F., Staiano M., Pelella F., Rossi M., Scire A., Tanfani F., Bertoli E., Grycznyski Z., Lakowicz JR., null, Structural and thermal stability characterization of Escherichia coli D-galactose/D-glucose-binding protein., Biotechnol Prog 20(1):330-7
[6] D'Auria S., Ausili A., Marabotti A., Varriale A., Scognamiglio V., Staiano M., Bertoli E., Rossi M., Tanfani F., 2006, Binding of glucose to the D-galactose/D-glucose-binding protein from Escherichia coli restores the native protein secondary structure and thermostability that are lost upon calcium depletion., J Biochem 139(2):213-21
[7] D'Auria S., Varriale A., Gonnelli M., Saviano M., Staiano M., Rossi M., Strambini GB., 2007, Tryptophan phosphorescence studies of the D-galactose/D-glucose-binding protein from Escherichia coli provide a molecular portrait with structural and dynamics features of the protein., J Proteome Res 6(4):1306-12
[8] Hazelbauer GL., Mesibov RE., Adler J., 1969, Escherichia coli mutants defective in chemotaxis toward specific chemicals., Proc Natl Acad Sci U S A 64(4):1300-7
[9] Herman P., Vecer J., Barvik I., Scognamiglio V., Staiano M., de Champdore M., Varriale A., Rossi M., D'Auria S., 2005, The role of calcium in the conformational dynamics and thermal stability of the D-galactose/D-glucose-binding protein from Escherichia coli., Proteins 61(1):184-95
[10] Kim M., Cho AE., 2016, The role of water molecules in stereoselectivity of glucose/galactose-binding protein., Sci Rep 6:36807
[11] Luck LA., Falke JJ., 1991, Open conformation of a substrate-binding cleft: 19F NMR studies of cleft angle in the D-galactose chemosensory receptor., Biochemistry 30(26):6484-90
[12] Miller DM., Olson JS., Pflugrath JW., Quiocho FA., 1983, Rates of ligand binding to periplasmic proteins involved in bacterial transport and chemotaxis., J Biol Chem 258(22):13665-72
[13] Miller DM., Olson JS., Quiocho FA., 1980, The mechanism of sugar binding to the periplasmic receptor for galactose chemotaxis and transport in Escherichia coli., J Biol Chem 255(6):2465-71
[14] Mowbray SL., 1992, Ribose and glucose-galactose receptors. Competitors in bacterial chemotaxis., J Mol Biol 227(2):418-40
[15] Muller N., Heine HG., Boos W., 1982, Cloning of mglB, the structural gene for the galactose-binding protein of Salmonella typhimurium and Escherichia coli., Mol Gen Genet 185(3):473-80
[16] Ortega G., Castano D., Diercks T., Millet O., 2012, Carbohydrate affinity for the glucose-galactose binding protein is regulated by allosteric domain motions., J Am Chem Soc 134(48):19869-76
[17] Park C., Hazelbauer GL., 1986, Mutations specifically affecting ligand interaction of the Trg chemosensory transducer., J Bacteriol 167(1):101-9
[18] Parsons RG., Hogg RW., 1974, A comparison of the L-arabinose- and D-galactose-binding proteins of Escherichia coli B-r., J Biol Chem 249(11):3608-14
[19] Piszczek G., D'Auria S., Staiano M., Rossi M., Ginsburg A., 2004, Conformational stability and domain coupling in D-glucose/D-galactose-binding protein from Escherichia coli., Biochem J 381(Pt 1):97-103
[20] Quiocho FA., Meador WE., Pflugrath JW., 1979, Preliminary crystallographic data of receptors for transport and chemotaxis in Escherichia coli: D-galactose and maltose-binding proteins., J Mol Biol 133(1):181-4
[21] Richarme G., 1983, Associative properties of the Escherichia coli galactose-binding protein and maltose-binding protein., Biochim Biophys Acta 748(1):99-108
[22] Scholle A., Vreemann J., Blank V., Nold A., Boos W., Manson MD., 1987, Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors., Mol Gen Genet 208(1-2):247-53
[23] Scognamiglio V., Scire A., Aurilia V., Staiano M., Crescenzo R., Palmucci C., Bertoli E., Rossi M., Tanfani F., D'Auria S., 2007, A strategic fluorescence labeling of D-galactose/D-glucose-binding protein from Escherichia coli helps to shed light on the protein structural stability and dynamics., J Proteome Res 6(11):4119-26
[24] Shilton BH., Flocco MM., Nilsson M., Mowbray SL., 1996, Conformational changes of three periplasmic receptors for bacterial chemotaxis and transport: the maltose-, glucose/galactose- and ribose-binding proteins., J Mol Biol 264(2):350-63
[25] Vyas NK., Vyas MN., Quiocho FA., 1991, Comparison of the periplasmic receptors for L-arabinose, D-glucose/D-galactose, and D-ribose. Structural and Functional Similarity., J Biol Chem 266(8):5226-37
[26] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78