RegulonDB RegulonDB 10.9: Gene Form
   

mrcB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mrcB hrpB fhuA Fur fhuAp fhuAp mrcBp mrcBp TSS_288 TSS_288

Gene      
Name: mrcB    Texpresso search in the literature
Synonym(s): ECK0148, EG10605, b0149, pbpF, ponB
Genome position(nucleotides): 164730 --> 167264 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.15
External database links:  
ASAP:
ABE-0000516
CGSC:
483
ECHOBASE:
EB0600
ECOLIHUB:
mrcB
OU-MICROARRAY:
b0149
STRING:
511145.b0149
COLOMBOS: mrcB


Product      
Name: peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcB
Synonym(s): MrcB, PBP1B, PBP1Balpha;, PBP1Bs, PbpF, PonB, penicillin binding protein 1B, penicillin binding protein 1Balpha;
Sequence: Get amino acid sequence Fasta Format
Cellular location: cell wall,inner membrane,periplasmic space
Molecular weight: 94.292
Isoelectric point: 9.458
Motif(s):
 
Type Positions Sequence
64 -> 87 GWLWLLLKLAIVFAVLIAIYGVYL
234 -> 234 D
303 -> 312 KDRILELYMN
355 -> 355 K
372 -> 377 RRNLVL

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.7 - murein (peptidoglycan)
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.12 - amino sugar conversions
  5 - cell processes --> 5.1 - cell division
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.2 - murein
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.7 - murein (peptidoglycan)
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.12 - amino sugar conversions
  5 - cell processes --> 5.1 - cell division
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.2 - murein
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0009274 - peptidoglycan-based cell wall
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016757 - transferase activity, transferring glycosyl groups
GO:0008233 - peptidase activity
GO:0016787 - hydrolase activity
GO:0016740 - transferase activity
GO:0004180 - carboxypeptidase activity
GO:0008955 - peptidoglycan glycosyltransferase activity
GO:0009002 - serine-type D-Ala-D-Ala carboxypeptidase activity
GO:0008144 - drug binding
GO:0008658 - penicillin binding
GO:0071723 - lipopeptide binding
biological_process GO:0010165 - response to X-ray
GO:0051518 - positive regulation of bipolar cell growth
GO:0071433 - cell wall repair
GO:0008152 - metabolic process
GO:0006508 - proteolysis
GO:0009252 - peptidoglycan biosynthetic process
GO:0046677 - response to antibiotic
GO:0008360 - regulation of cell shape
GO:0071555 - cell wall organization
Reference(s): [1] de la Rosa EJ., et al., 1985
External database links:  
CAZY:
GT51
DIP:
DIP-10252N
ECOCYC:
EG10605-MONOMER
ECOLIWIKI:
b0149
INTERPRO:
IPR012338
INTERPRO:
IPR001264
INTERPRO:
IPR001460
INTERPRO:
IPR023346
INTERPRO:
IPR028166
INTERPRO:
IPR032730
INTERPRO:
IPR036950
INTERPRO:
IPR011813
MODBASE:
P02919
PDB:
6G5R
PDB:
6FZK
PDB:
5HLD
PDB:
5HLB
PDB:
5HLA
PDB:
5HL9
PDB:
5FGZ
PDB:
3VMA
PDB:
3FWL
PFAM:
PF00905
PFAM:
PF14814
PFAM:
PF14812
PFAM:
PF00912
PRIDE:
P02919
PRODB:
PRO_000023286
REFSEQ:
NP_414691
SMR:
P02919
UNIPROT:
P02919


Operon      
Name: mrcB         
Operon arrangement:
Transcription unit        Promoter
mrcB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_288 164658 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] de la Rosa EJ., de Pedro MA., Vazquez D., 1985, Penicillin binding proteins: role in initiation of murein synthesis in Escherichia coli., Proc Natl Acad Sci U S A 82(17):5632-5

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


RegulonDB