RegulonDB RegulonDB 10.9: Gene Form
   

mrdB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mrdA mrdB rlpA TSS_759 TSS_759 TSS_758 TSS_758 TSS_757 TSS_757 rlpAp5 rlpAp5 rlpAp7 rlpAp7

Gene      
Name: mrdB    Texpresso search in the literature
Synonym(s): ECK0627, EG10607, b0634, rodA
Genome position(nucleotides): 665201 <-- 666313 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.02
External database links:  
ASAP:
 ABE-0002173
CGSC:
 18145
ECHOBASE:
 EB0602
ECOLIHUB:
 mrdB
OU-MICROARRAY:
 b0634
STRING:
 511145.b0634
COLOMBOS: mrdB


Product      
Name: SEDS family protein MrdB
Synonym(s): MrdB, RodA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 40.476
Isoelectric point: 9.663
Motif(s):
 
Type Positions Sequence
117 -> 117 K
262 -> 262 D
114 -> 114 E
159 -> 159 D
160 -> 180 LGTSILVALSGLFVLFLSGLS

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.9.B - Putative uncharacterized transport protein
  5 - cell processes --> 5.1 - cell division
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0032153 - cell division site
molecular_function GO:0005515 - protein binding
GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016740 - transferase activity
GO:0008955 - peptidoglycan glycosyltransferase activity
GO:0015648 - lipid-linked peptidoglycan transporter activity
biological_process GO:0051301 - cell division
GO:0009252 - peptidoglycan biosynthetic process
GO:0008360 - regulation of cell shape
GO:0071555 - cell wall organization
GO:0015836 - lipid-linked peptidoglycan transport
Note(s): Note(s): ...[more].
Evidence: [AUP] Assay of unpurified protein
[IGI] Inferred from genetic interaction
[IHBCE] Inferred by a human based on computational evidence
Reference(s): [1] Begg KJ., et al., 1985
[2] Begg KJ., et al., 1998
[3] Begg KJ., et al., 1986
[4] Begg KJ., et al., 1990
[5] Bylund JE., et al., 1991
[6] Cho H., et al., 2016
[7] Garcia del Portillo F., et al., 1991
[8] Ishino F., et al., 1986
[9] Matsuhashi M., et al., 1990
[10] Meeske AJ., et al., 2016
[11] Shiomi D., et al., 2013
[12] Spratt BG., et al., 1980
[13] Tamaki S., et al., 1980
[14] Vinella D., et al., 1993
[15] de Pedro MA., et al., 2001
External database links:  
DIP:
 DIP-48062N
ECOCYC:
 EG10607-MONOMER
ECOLIWIKI:
 b0634
INTERPRO:
 IPR018365
INTERPRO:
 IPR011923
INTERPRO:
 IPR001182
PANTHER:
 PTHR30474:SF1
PANTHER:
 PTHR30474
PFAM:
 PF01098
PRIDE:
 P0ABG7
PRODB:
 PRO_000023288
PROSITE:
 PS00428
REFSEQ:
 NP_415167
UNIPROT:
 P0ABG7


Operon      
Name: rsfS-rlmH-mrdAB-rlpA         
Operon arrangement:
Transcription unit        Promoter
rsfS-rlmH-mrdAB-rlpA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter rlpAp7 665213 reverse Similarity to the consensus
Read more > 		[ICWHO] [16]
  promoter rlpAp5 665222 reverse Similarity to the consensus
Read more > 		[ICWHO] [16]
  promoter TSS_757 665332 reverse nd [RS-EPT-CBR] [17]
  promoter TSS_758 665461 reverse nd [RS-EPT-CBR] [17]
  promoter TSS_759 667132 reverse nd [RS-EPT-CBR] [17]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Begg KJ., Donachie WD., 1985, Cell shape and division in Escherichia coli: experiments with shape and division mutants., J Bacteriol 163(2):615-22

 [2] Begg KJ., Donachie WD., 1998, Division planes alternate in spherical cells of Escherichia coli., J Bacteriol 180(9):2564-7

 [3] Begg KJ., Spratt BG., Donachie WD., 1986, Interaction between membrane proteins PBP3 and rodA is required for normal cell shape and division in Escherichia coli., J Bacteriol 167(3):1004-8

 [4] Begg KJ., Takasuga A., Edwards DH., Dewar SJ., Spratt BG., Adachi H., Ohta T., Matsuzawa H., Donachie WD., 1990, The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide., J Bacteriol 172(12):6697-703

 [5] Bylund JE., Haines MA., Walsh K., Bouloc P., D'Ari R., Higgins ML., 1991, Buoyant density studies of several mecillinam-resistant and division mutants of Escherichia coli., J Bacteriol 173(17):5396-402

 [6] Cho H., Wivagg CN., Kapoor M., Barry Z., Rohs PDA., Suh H., Marto JA., Garner EC., Bernhardt TG., 2016, Bacterial cell wall biogenesis is mediated by SEDS and PBP polymerase families functioning semi-autonomously., Nat Microbiol 1:16172

 [7] Garcia del Portillo F., de Pedro MA., 1991, Penicillin-binding protein 2 is essential for the integrity of growing cells of Escherichia coli ponB strains., J Bacteriol 173(14):4530-2

 [8] Ishino F., Park W., Tomioka S., Tamaki S., Takase I., Kunugita K., Matsuzawa H., Asoh S., Ohta T., Spratt BG., 1986, Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and rodA protein., J Biol Chem 261(15):7024-31

 [9] Matsuhashi M., Wachi M., Ishino F., 1990, Machinery for cell growth and division: penicillin-binding proteins and other proteins., Res Microbiol 141(1):89-103

 [10] Meeske AJ., Riley EP., Robins WP., Uehara T., Mekalanos JJ., Kahne D., Walker S., Kruse AC., Bernhardt TG., Rudner DZ., 2016, SEDS proteins are a widespread family of bacterial cell wall polymerases., Nature 537(7622):634-638

 [11] Shiomi D., Toyoda A., Aizu T., Ejima F., Fujiyama A., Shini T., Kohara Y., Niki H., 2013, Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells., Mol Microbiol 87(5):1029-44

 [12] Spratt BG., Boyd A., Stoker N., 1980, Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome., J Bacteriol 143(2):569-81

 [13] Tamaki S., Matsuzawa H., Matsuhashi M., 1980, Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli., J Bacteriol 141(1):52-7

 [14] Vinella D., Joseleau-Petit D., Thevenet D., Bouloc P., D'Ari R., 1993, Penicillin-binding protein 2 inactivation in Escherichia coli results in cell division inhibition, which is relieved by FtsZ overexpression., J Bacteriol 175(20):6704-10

 [15] de Pedro MA., Donachie WD., Holtje JV., Schwarz H., 2001, Constitutive septal murein synthesis in Escherichia coli with impaired activity of the morphogenetic proteins RodA and penicillin-binding protein 2., J Bacteriol 183(14):4115-26

 [16] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [17] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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