RegulonDB RegulonDB 11.1: Gene Form
   

lpxM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pykA mepM lpxM anti-anti-terminator anti-terminator terminator TSS_2210 TSS_2210 TSS_2209 TSS_2209 lpxMp4 lpxMp4 TSS_2208 TSS_2208 TSS_2207 TSS_2207 TSS_2206 TSS_2206 TSS_2205 TSS_2205

Gene      
Name: lpxM    Texpresso search in the literature
Synonym(s): ECK1856, EG10614, b1855, mlt, msbB, waaN
Genome position(nucleotides): 1939222 <-- 1940193
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.54
External database links:  
ASAP:
 ABE-0006185
CGSC:
 32359
ECHOBASE:
 EB0609
ECOLIHUB:
 lpxM
OU-MICROARRAY:
 b1855
STRING:
 511145.b1855
COLOMBOS: lpxM


Product      
Name: Lipid A biosynthesis myristoyltransferase
Synonym(s): LpxM, Mlt, MsbB, WaaN, myristoyl-acyl carrier protein-dependent acyltransferase
Sequence: Get amino acid sequence Fasta Format
Cellular location: cell wall,inner membrane
Molecular weight: 37.41
Isoelectric point: 10.058
Motif(s):
 
Type Positions Sequence Comment
12 -> 305 PEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAMFHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARRMNEEVEIFVGPRPEQYTWILKLLKT
23 -> 43 YWGAWLGVAAMAGIALTPPKF UniProt: Helical.
139 -> 144 HGWAVD UniProt: HXXXXD motif; Sequence Annotation Type: short sequence motif.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.3 - lipid A
  6 - cell structure --> 6.1 - membrane
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0009276 - Gram-negative-bacterium-type cell wall
molecular_function GO:0016740 - transferase activity
GO:0016746 - acyltransferase activity
GO:0016747 - acyltransferase activity, transferring groups other than amino-acyl groups
GO:0019107 - myristoyltransferase activity
biological_process GO:0009103 - lipopolysaccharide biosynthetic process
GO:0036104 - Kdo2-lipid A biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P24205
DIP:
 DIP-10262N
ECOCYC:
 MYRISTOYLACYLTRAN-MONOMER
ECOLIWIKI:
 b1855
INTERPRO:
 IPR004960
INTERPRO:
 IPR011921
PANTHER:
 PTHR30606
PFAM:
 PF03279
PRIDE:
 P24205
PRODB:
 PRO_000023295
REFSEQ:
 NP_416369
SMR:
 P24205
UNIPROT:
 P24205


Operon      
Name: lpxM         
Operon arrangement:
Transcription unit        Promoter
lpxM


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2205 1937670 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2206 1937972 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2207 1938121 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2208 1938452 forward nd [RS-EPT-CBR] [1]
  promoter lpxMp4 1940228 reverse nd [COMP-AINF] [2]
  promoter TSS_2209 1940588 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2210 1940853 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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