RegulonDB RegulonDB 11.0: Gene Form
   

mutH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ygdQ mutH ygdT TSS_3132 TSS_3132 ygdQp3 ygdQp3 mutHp7 mutHp7 mutHp5 mutHp5

Gene      
Name: mutH    Texpresso search in the literature
Synonym(s): ECK2827, EG10624, b2831, mutR, prv
Genome position(nucleotides): 2969662 --> 2970351
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.62
External database links:  
ASAP:
 ABE-0009287
CGSC:
 471
ECHOBASE:
 EB0619
ECOLIHUB:
 mutH
OU-MICROARRAY:
 b2831
STRING:
 511145.b2831
COLOMBOS: mutH


Product      
Name: DNA mismatch repair protein MutH
Synonym(s): MutH, MutR, Prv
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 25.527
Isoelectric point: 6.971
Motif(s):
 
Type Positions Sequence Comment
2 -> 229 SQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALVGLVTPENLKRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTGNSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEELMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFTSALLARHFLIQ UniProt: DNA mismatch repair protein MutH.
28 -> 28 T UniProt: In TS7; temperature-sensitive..
58 -> 58 W UniProt: In strain: ECOR 67..
59 -> 157 LGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTGNSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLRED
60 -> 60 G UniProt: In strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.1 - DNA related --> 2.1.4 - DNA repair
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0032300 - mismatch repair complex
molecular_function GO:0003677 - DNA binding
GO:0004518 - nuclease activity
GO:0004519 - endonuclease activity
GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0043765 - T/G mismatch-specific endonuclease activity
biological_process GO:0006281 - DNA repair
GO:0006304 - DNA modification
GO:0006974 - cellular response to DNA damage stimulus
GO:0006298 - mismatch repair
GO:0090305 - nucleic acid phosphodiester bond hydrolysis
GO:0000018 - regulation of DNA recombination
Reference(s): [1] Au KG., et al., 1992
[2] Ban C., et al., 1998
[3] Tsui HC., et al., 1997
[4] Welsh KM., et al., 1987
External database links:  
ALPHAFOLD:
 P06722
DIP:
 DIP-10284N
ECOCYC:
 EG10624-MONOMER
ECOLIWIKI:
 b2831
INTERPRO:
 IPR011337
INTERPRO:
 IPR037057
INTERPRO:
 IPR011335
INTERPRO:
 IPR004230
MODBASE:
 P06722
PDB:
 2AZO
PDB:
 1AZO
PFAM:
 PF02976
PRIDE:
 P06722
PRODB:
 PRO_000023323
REFSEQ:
 NP_417308
SMART:
 SM00927
SMR:
 P06722
UNIPROT:
 P06722


Operon      
Name: mutH         
Operon arrangement:
Transcription unit        Promoter
mutH


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter mutHp5 2969582 forward nd [ICWHO] [5]
  promoter mutHp7 2969614 forward nd [ICWHO] [5]
  promoter ygdQp3 2970392 forward nd [ICWHO] [5]
  promoter TSS_3132 2970394 forward nd [RS-EPT-CBR] [6]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Au KG., Welsh K., Modrich P., 1992, Initiation of methyl-directed mismatch repair., J Biol Chem 267(17):12142-8

 [2] Ban C., Yang W., 1998, Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases., EMBO J 17(5):1526-34

 [3] Tsui HC., Feng G., Winkler ME., 1997, Negative regulation of mutS and mutH repair gene expression by the Hfq and RpoS global regulators of Escherichia coli K-12., J Bacteriol 179(23):7476-87

 [4] Welsh KM., Lu AL., Clark S., Modrich P., 1987, Isolation and characterization of the Escherichia coli mutH gene product., J Biol Chem 262(32):15624-9

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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