RegulonDB RegulonDB 11.1: Gene Form
   

emrE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

insEF3 insF3 emrE ylcJ insE3 terminator anti-terminator ybcKp5 ybcKp5 ybcKp1 ybcKp1 ybcKp7 ybcKp7

Gene      
Name: emrE    Texpresso search in the literature
Synonym(s): ECK0534, EG10629, b0543, eb, mvrC
Genome position(nucleotides): 568315 --> 568647
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 41.14
External database links:  
ASAP:
 ABE-0001863
CGSC:
 36935
ECHOBASE:
 EB0623
ECOLIHUB:
 emrE
OU-MICROARRAY:
 b0543
STRING:
 511145.b0543
COLOMBOS: emrE


Product      
Name: DLP12 prophage; multidrug/betaine/choline efflux transporter EmrE
Synonym(s): EBR, EmrE, MvrC
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 11.958
Isoelectric point: 8.039
Motif(s):
 
Type Positions Sequence Comment
3 -> 94 PYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASFWLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLI
4 -> 4 Y UniProt: Still binds substrate. No transport activity in the presence of a proton gradient, but still transports substrate in the absence of a proton gradient. Resistance to toxicants is abolished..
6 -> 6 Y UniProt: No effect on resistance to toxicants..
7 -> 7 L UniProt: No substrate binding. Resistance to toxicants is abolished..
10 -> 10 A UniProt: Still binds substrate, with lower affinity. Resistance to toxicants is abolished..

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:1990207 - EmrE multidrug transporter complex
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015297 - antiporter activity
GO:0022857 - transmembrane transporter activity
GO:0015299 - solute:proton antiporter activity
GO:0042802 - identical protein binding
GO:0042910 - xenobiotic transmembrane transporter activity
GO:0015199 - amino-acid betaine transmembrane transporter activity
GO:0015220 - choline transmembrane transporter activity
biological_process GO:1990961 - xenobiotic detoxification by transmembrane export across the plasma membrane
GO:0006805 - xenobiotic metabolic process
GO:0006970 - response to osmotic stress
GO:0006974 - cellular response to DNA damage stimulus
GO:0009410 - response to xenobiotic stimulus
GO:0055085 - transmembrane transport
GO:0042908 - xenobiotic transport
GO:0031460 - glycine betaine transport
GO:0015871 - choline transport
External database links:  
ALPHAFOLD:
 P23895
DIP:
 DIP-9505N
ECOCYC:
 EMRE-MONOMER
ECOLIWIKI:
 b0543
INTERPRO:
 IPR000390
MODBASE:
 P23895
PANTHER:
 PTHR30561
PDB:
 3B61
PDB:
 3B5D
PDB:
 3B62
PDB:
 2I68
PFAM:
 PF00893
PRIDE:
 P23895
PRODB:
 PRO_000022512
REFSEQ:
 NP_415075
SMR:
 P23895
UNIPROT:
 P23895


Operon      
Name: emrE         
Operon arrangement:
Transcription unit        Promoter
emrE


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybcKp7 568751 forward nd [COMP-AINF] [1]
  promoter ybcKp1 568787 forward nd [COMP-AINF] [1]
  promoter ybcKp5 568810 forward nd [COMP-AINF] [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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