RegulonDB RegulonDB 10.9: Gene Form
   

nanA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nanT nanA nanR CRP Fis Fis NanR NanR NanR Fis terminator nanAp nanAp

Gene      
Name: nanA    Texpresso search in the literature
Synonym(s): ECK3214, EG10637, b3225, npl
Genome position(nucleotides): 3372683 <-- 3373576 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.01
External database links:  
ASAP:
ABE-0010579
CGSC:
17647
ECHOBASE:
EB0631
ECOLIHUB:
nanA
OU-MICROARRAY:
b3225
STRING:
511145.b3225
COLOMBOS: nanA


Product      
Name: N-acetylneuraminate lyase
Synonym(s): NanA, Npl
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.593
Isoelectric point: 5.69
Motif(s):
 
Type Positions Sequence
70 -> 70 A
282 -> 282 L
5 -> 291 LRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYVGGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDAVSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPGVGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQGIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGPVDEKYLPELKALAQQ
84 -> 84 S
2 -> 297 ATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYVGGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDAVSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPGVGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQGIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGPVDEKYLPELKALAQQLMQERG

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.12 - amino sugar conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0008747 - N-acetylneuraminate lyase activity
GO:0042802 - identical protein binding
biological_process GO:0005975 - carbohydrate metabolic process
GO:0019262 - N-acetylneuraminate catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Owen RL., et al., 2012
[2] Wang TH., et al., 2009
External database links:  
DIP:
DIP-10302N
ECOCYC:
ACNEULY-MONOMER
ECOLIWIKI:
b3225
INTERPRO:
IPR005264
INTERPRO:
IPR013785
INTERPRO:
IPR020624
INTERPRO:
IPR020625
INTERPRO:
IPR002220
MODBASE:
P0A6L4
PANTHER:
PTHR42849
PDB:
2YGY
PDB:
3LBC
PDB:
3LBM
PDB:
3LCF
PDB:
3LCG
PDB:
3LCH
PDB:
3LCI
PDB:
3LCL
PDB:
3LCW
PDB:
3LCX
PDB:
4BWL
PDB:
4UUI
PDB:
2XFW
PDB:
2WPB
PDB:
2WO5
PDB:
2WNZ
PDB:
2WNQ
PDB:
2WNN
PDB:
2WKJ
PDB:
1NAL
PDB:
1HL2
PDB:
1FDZ
PDB:
1FDY
PFAM:
PF00701
PRIDE:
P0A6L4
PRINTS:
PR00146
PROSITE:
PS00665
PROSITE:
PS00666
REFSEQ:
NP_417692
SMART:
SM01130
SMR:
P0A6L4
UNIPROT:
P0A6L4


Operon      
Name: nanATEK-yhcH         
Operon arrangement:
Transcription unit        Promoter
nanA
nanATEK-yhcH


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: NanR, Fis


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Owen RL., Yorke BA., Pearson AR., 2012, X-ray-excited optical luminescence of protein crystals: a new tool for studying radiation damage during diffraction data collection., Acta Crystallogr D Biol Crystallogr 68(Pt 5):505-10

 [2] Wang TH., Chen YY., Pan HH., Wang FP., Cheng CH., Lee WC., 2009, Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins., BMC Biotechnol 9:63


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