RegulonDB RegulonDB 11.1: Gene Form
   

narK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

narG narX narK narS narL narH FNR NarL Fis NarL NarL NarL NarL Fis IHF NarL NarL NarL RstA ModE FNR Fis FNR NarL NarL FNR FNR NarL NarL NarL IHF NarL NarL NarL NarL NarL FNR TSS_1555 TSS_1555 narGp narGp narKp1 narKp1 narKp2 narKp2 narXp narXp

Gene      
Name: narK    Texpresso search in the literature
Synonym(s): ECK1217, EG10642, b1223
Genome position(nucleotides): 1277957 --> 1279348
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.66
External database links:  
ASAP:
 ABE-0004111
CGSC:
 18112
ECHOBASE:
 EB0636
ECOLIHUB:
 narK
OU-MICROARRAY:
 b1223
STRING:
 511145.b1223
COLOMBOS: narK


Product      
Name: nitrate:nitrite antiporter NarK
Synonym(s): NarK
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 49.693
Isoelectric point: 10.131
Motif(s):
 
Type Positions Sequence Comment
38 -> 58 LWISVPCLLLAFCVWMLFSAV UniProt: Helical.
45 -> 422 LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGRRWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPKQKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWIWVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGLLFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAATDTAAALGFISAIGAIGGFFIP
79 -> 99 ALPSVSGALLRVPYSFMVPIF UniProt: Helical.
104 -> 124 WTAFSTGILIIPCVWLGFAVQ UniProt: Helical.
132 -> 152 VFIIISLLCGFAGANFASSMA UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.3 - nitrogen metabolism
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005452 - inorganic anion exchanger activity
GO:0015112 - nitrate transmembrane transporter activity
GO:0015113 - nitrite transmembrane transporter activity
GO:0015513 - high-affinity secondary active nitrite transmembrane transporter activity
GO:0015514 - nitrite efflux transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
biological_process GO:0015706 - nitrate transport
GO:0015707 - nitrite transport
GO:0042128 - nitrate assimilation
GO:0055085 - transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Bonnefoy V., et al., 1997
[2] Miller JB., et al., 1983
[3] Stewart V., et al., 1988
External database links:  
ALPHAFOLD:
 P10903
ECOCYC:
 NARK-MONOMER
ECOLIWIKI:
 b1223
INTERPRO:
 IPR036259
INTERPRO:
 IPR011701
INTERPRO:
 IPR004737
MODBASE:
 P10903
PDB:
 4U4W
PDB:
 4U4V
PDB:
 4U4T
PDB:
 4JR9
PDB:
 4JRE
PFAM:
 PF07690
PRIDE:
 P10903
PRODB:
 PRO_000023355
REFSEQ:
 NP_415741
SMR:
 P10903
UNIPROT:
 P10903


Operon      
Name: narKS         
Operon arrangement:
Transcription unit        Promoter
narK
narK


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, IHF, NarL
Repressed by: Fis


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1555 1280972 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Bonnefoy V., Ratouchniak J., Blasco F., Chippaux M., 1997, Organization of the nar genes at the chlZ locus., FEMS Microbiol Lett 147(1):147-9

 [2] Miller JB., Amy NK., 1983, Molybdenum cofactor in chlorate-resistant and nitrate reductase-deficient insertion mutants of Escherichia coli., J Bacteriol 155(2):793-801

 [3] Stewart V., Berg BL., 1988, Influence of nar (nitrate reductase) genes on nitrate inhibition of formate-hydrogen lyase and fumarate reductase gene expression in Escherichia coli K-12., J Bacteriol 170(10):4437-44

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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