RegulonDB

RegulonDB RegulonDB 11.1: Gene Form

narL gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	narL
Synonym(s):	ECK1215, EG10643, b1221, frdR, narR
Genome position(nucleotides):	1275179 <-- 1275829
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.77
External database links:

ASAP:	ABE-0004097
CGSC:	18109
ECHOBASE:	EB0637
ECOLIHUB:	narL
OU-MICROARRAY:	b1221
STRING:	511145.b1221
COLOMBOS:	narL

Shine dalgarno
Sequence:	acagacgtccAAGGAGataccCAT

Product

Name:

DNA-binding transcriptional dual regulator NarL

Synonym(s):

FrdR, NarL, NarR, nitrate/nitrite response regulator NarL

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

LuxR/UhpA

Cellular location:

cytosol

Molecular weight:

23.927

Isoelectric point:

5.97

Motif(s):

Type	Positions	Sequence	Comment
	8 -> 124	TILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQGIELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRGADGYLLKDMEPEDLLKALHQAA	UniProt: Response regulatory.
	9 -> 120	ILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQGIELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRGADGYLLKDMEPEDLLKAL
	115 -> 115	D	UniProt: In Ref. 2; AAA24199..
	149 -> 214	TERDVNQLTPRERDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQER	UniProt: HTH luxR-type.
	155 -> 209	QLTPRERDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVW

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.4 - complex regulation --> 3.1.2.4.3 - two component regulatory systems (external signal)
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0032993 - protein-DNA complex
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0000166 - nucleotide binding GO:0005524 - ATP binding GO:0042802 - identical protein binding GO:0000976 - transcription cis-regulatory region binding GO:0001217 - DNA-binding transcription repressor activity
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0045892 - negative regulation of transcription, DNA-templated GO:0000160 - phosphorelay signal transduction system GO:0042128 - nitrate assimilation

Evidence:

[COMP-AINF-FN-FROM-SEQ] Automated inference of function from sequence

Reference(s):

[1] Darwin AJ., et al., 1995
[2] Egan SM., et al., 1991
[3] Gunsalus RP., et al., 1989
[4] Nohno T., et al., 1989
[5] Pao GM., et al., 1995
[6] Parkinson JS. 1993
[7] Parkinson JS., et al., 1992
[8] Rabin RS., et al., 1993
[9] Stewart V., et al., 1989
[10] Stock JB., et al., 1990
[11] Tyson KL., et al., 1994

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMART:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

narXL

Operon arrangement:

Transcription unit	Promoter
narXL	narXp

Transcriptional Regulation
	Display Regulation
Activated by:	ModE
Repressed by:	FNR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Darwin AJ., Stewart V., 1995, Nitrate and nitrite regulation of the Fnr-dependent aeg-46.5 promoter of Escherichia coli K-12 is mediated by competition between homologous response regulators (NarL and NarP) for a common DNA-binding site., J Mol Biol 251(1):15-29
[2] Egan SM., Stewart V., 1991, Mutational analysis of nitrate regulatory gene narL in Escherichia coli K-12., J Bacteriol 173(14):4424-32
[3] Gunsalus RP., Kalman LV., Stewart RR., 1989, Nucleotide sequence of the narL gene that is involved in global regulation of nitrate controlled respiratory genes of Escherichia coli., Nucleic Acids Res 17(5):1965-75
[4] Nohno T., Noji S., Taniguchi S., Saito T., 1989, The narX and narL genes encoding the nitrate-sensing regulators of Escherichia coli are homologous to a family of prokaryotic two-component regulatory genes., Nucleic Acids Res 17(8):2947-57
[5] Pao GM., Saier MH., 1995, Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution., J Mol Evol 40(2):136-54
[6] Parkinson JS., 1993, Signal transduction schemes of bacteria., Cell 73(5):857-71
[7] Parkinson JS., Kofoid EC., 1992, Communication modules in bacterial signaling proteins., Annu Rev Genet 26:71-112
[8] Rabin RS., Stewart V., 1993, Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12., J Bacteriol 175(11):3259-68
[9] Stewart V., Parales J., Merkel SM., 1989, Structure of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12., J Bacteriol 171(4):2229-34
[10] Stock JB., Stock AM., Mottonen JM., 1990, Signal transduction in bacteria., Nature 344(6265):395-400
[11] Tyson KL., Cole JA., Busby SJ., 1994, Nitrite and nitrate regulation at the promoters of two Escherichia coli operons encoding nitrite reductase: identification of common target heptamers for both NarP- and NarL-dependent regulation., Mol Microbiol 13(6):1045-55

RegulonDB

Contact us Funding Terms & conditions How to cite