RegulonDB RegulonDB 10.9: Gene Form
   

ompT gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ompT envY appY PhoP OmrB OmrA ompTp ompTp TSS_695 (cluster) TSS_695 (cluster) TSS_694 TSS_694 TSS_693 TSS_693 TSS_692 TSS_692 TSS_691 TSS_691 TSS_690 (cluster) TSS_690 (cluster) TSS_689 TSS_689 TSS_688 TSS_688 TSS_687 TSS_687

Gene      
Name: ompT    Texpresso search in the literature
Synonym(s): ECK0557, EG10673, b0565
Genome position(nucleotides): 584680 <-- 585633 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
43.4
External database links:  
ASAP:
ABE-0001931
CGSC:
4984
ECHOBASE:
EB0667
ECOLIHUB:
ompT
OU-MICROARRAY:
b0565
STRING:
511145.b0565
COLOMBOS: ompT


Product      
Name: DLP12 prophage; protease 7
Synonym(s): OmpT, omptin, outer membrane protease VII (outer membrane protein 3b)
Sequence: Get amino acid sequence Fasta Format
Cellular location: outer membrane
Molecular weight: 35.562
Isoelectric point: 5.83
Motif(s):
 
Type Positions Sequence
238 -> 238 R
265 -> 274 AKVYVEGAWN
21 -> 317 STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF
105 -> 105 D
28 -> 317 TPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.3 - proteins/peptides/glycopeptides
  6 - cell structure --> 6.1 - membrane
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
Gene Ontology Terms (GO)  
cellular_component GO:0009279 - cell outer membrane
GO:0016020 - membrane
GO:0016021 - integral component of membrane
GO:0031230 - intrinsic component of cell outer membrane
molecular_function GO:0008233 - peptidase activity
GO:0016787 - hydrolase activity
GO:0004175 - endopeptidase activity
GO:0004190 - aspartic-type endopeptidase activity
GO:0004252 - serine-type endopeptidase activity
biological_process GO:0006508 - proteolysis
Note(s): Note(s): ...[more].
Evidence: [IMP] Inferred from mutant phenotype
Reference(s): [1] Hamdallah I., et al., 2018
[2] Kukkonen M., et al., 2004
[3] Stathopoulos C. 1998
[4] Zhang DF., et al., 2018
External database links:  
ECOCYC:
EG10673-MONOMER
ECOLIWIKI:
b0565
INTERPRO:
IPR020080
INTERPRO:
IPR020079
INTERPRO:
IPR000036
MODBASE:
P09169
PDB:
1I78
PFAM:
PF01278
PRIDE:
P09169
PRINTS:
PR00482
PRODB:
PRO_000023458
PROSITE:
PS00835
PROSITE:
PS00834
REFSEQ:
NP_415097
SMR:
P09169
UNIPROT:
P09169


Operon      
Name: envY-ompT         
Operon arrangement:
Transcription unit        Promoter
ompT
envY-ompT


Transcriptional Regulation      
Display Regulation             
Activated by: PhoP


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_687 584165 forward nd [RS-EPT-CBR] [5]
  promoter TSS_688 585378 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_689 585380 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_690 (cluster) 585383 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [5]
  promoter TSS_691 585398 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_692 585408 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_693 585420 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_694 585594 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_695 (cluster) 585659 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Hamdallah I., Torok N., Bischof KM., Majdalani N., Chadalavada S., Mdluli N., Creamer KE., Clark M., Holdener C., Basting PJ., Gottesman S., Slonczewski JL., 2018, Experimental Evolution of Escherichia coli K-12 at High pH and with RpoS Induction., Appl Environ Microbiol 84(15)

 [2] Kukkonen M., Korhonen TK., 2004, The omptin family of enterobacterial surface proteases/adhesins: from housekeeping in Escherichia coli to systemic spread of Yersinia pestis., Int J Med Microbiol 294(1):7-14

 [3] Stathopoulos C., 1998, Structural features, physiological roles, and biotechnological applications of the membrane proteases of the OmpT bacterial endopeptidase family: a micro-review., Membr Cell Biol 12(1):1-8

 [4] Zhang DF., Ye JZ., Dai HH., Lin XM., Li H., Peng XX., 2018, Identification of ethanol tolerant outer membrane proteome reveals OmpC-dependent mechanism in a manner of EnvZ/OmpR regulation in Escherichia coli., J Proteomics 179:92-99

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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