RegulonDB RegulonDB 11.1: Gene Form
   

pepA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pepA holC ytgB TSS_5108 TSS_5108 holCp holCp TSS_5107 TSS_5107 TSS_5106 TSS_5106 valSp1 valSp1 valSp2 valSp2

Gene      
Name: pepA    Texpresso search in the literature
Synonym(s): ECK4253, EG10694, b4260, carP, xerB
Genome position(nucleotides): 4484440 <-- 4485951
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.29
External database links:  
ASAP:
 ABE-0013953
CGSC:
 30215
ECHOBASE:
 EB0688
ECOLIHUB:
 pepA
OU-MICROARRAY:
 b4260
STRING:
 511145.b4260
COLOMBOS: pepA


Product      
Name: aminopeptidase A/I
Synonym(s): CarP, PepA, XerB
Sequence: Get amino acid sequence Fasta Format
Regulator Family: Aminopeptidase
Cellular location: cytosol
Molecular weight: 54.88
Isoelectric point: 7.249
Motif(s):
 
Type Positions Sequence Comment
19 -> 147 VVGVFEPRRLSPIAEQLDKISDGYISALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLNDTGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNK
186 -> 489 AAKDLGNMPPNICNAAYLASQARQLADSYSKNVITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDSGGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRPGDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHITGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITAGCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALL
354 -> 354 E UniProt: Loss of activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.3 - proteins/peptides/glycopeptides
  2 - information transfer --> 2.1 - DNA related --> 2.1.3 - DNA recombination
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  2 - information transfer --> 2.3 - protein related --> 2.3.6 - turnover, degradation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0008233 - peptidase activity
GO:0016787 - hydrolase activity
GO:0046872 - metal ion binding
GO:0004177 - aminopeptidase activity
GO:0008235 - metalloexopeptidase activity
GO:0030145 - manganese ion binding
GO:0070006 - metalloaminopeptidase activity
GO:0001073 - transcription antitermination factor activity, DNA binding
biological_process GO:0006276 - plasmid maintenance
GO:0006351 - transcription, DNA-templated
GO:0006508 - proteolysis
GO:0019538 - protein metabolic process
GO:0031564 - transcription antitermination
GO:0042150 - plasmid recombination
GO:0043171 - peptide catabolic process
Reference(s): [1] Devroede N., et al., 2004
External database links:  
ALPHAFOLD:
 P68767
DIP:
 DIP-47860N
ECOCYC:
 EG10694-MONOMER
ECOLIWIKI:
 b4260
INTERPRO:
 IPR043472
INTERPRO:
 IPR023042
INTERPRO:
 IPR011356
INTERPRO:
 IPR008283
INTERPRO:
 IPR000819
MODBASE:
 P68767
PANTHER:
 PTHR11963
PDB:
 1GYT
PFAM:
 PF02789
PFAM:
 PF00883
PRIDE:
 P68767
PRINTS:
 PR00481
PROSITE:
 PS00631
REFSEQ:
 NP_418681
SMR:
 P68767
UNIPROT:
 P68767


Operon      
Name: pepA         
Operon arrangement:
Transcription unit        Promoter
pepA
pepA
pepA


Transcriptional Regulation      
Display Regulation             
Repressed by: PepA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_5106 4483936 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_5107 4483996 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_5108 4486040 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Devroede N., Thia-Toong TL., Gigot D., Maes D., Charlier D., 2004, Purine and pyrimidine-specific repression of the Escherichia coli carAB operon are functionally and structurally coupled., J Mol Biol 336(1):25-42

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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