RegulonDB

Gene
Name:	phoB
Synonym(s):	ECK0393, EG10728, b0399, phoRc, phoT
Genome position(nucleotides):	417142 --> 417831
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.49
External database links:

ASAP:	ABE-0001389
CGSC:	397
ECHOBASE:	EB0721
ECOLIHUB:	phoB
OU-MICROARRAY:	b0399
STRING:	511145.b0399
COLOMBOS:	phoB

Product

Name:

DNA-binding transcriptional dual regulator PhoB

Synonym(s):

PhoB, PhoB response regulator, PhoT

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

OmpR

Cellular location:

cytosol

Molecular weight:

26.433

Isoelectric point:

5.41

Motif(s):

Type	Positions	Sequence	Comment
	4 -> 120	RILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM	UniProt: Response regulatory.
	5 -> 117	ILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIK
	129 -> 227	EEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEFKLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFST	UniProt: OmpR/PhoB-type.
	151 -> 225	EPLEMGPTEFKLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRF

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.1 - phosphorous metabolism
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.4 - complex regulation --> 3.1.2.4.3 - two component regulatory systems (external signal)
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.2 - regulon
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0032993 - protein-DNA complex
	molecular_function	GO:0003677 - DNA binding GO:0000156 - phosphorelay response regulator activity GO:0042802 - identical protein binding GO:0000976 - transcription cis-regulatory region binding GO:0001108 - bacterial-type RNA polymerase holo enzyme binding GO:0001216 - DNA-binding transcription activator activity
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0045893 - positive regulation of transcription, DNA-templated GO:0000160 - phosphorelay signal transduction system GO:0006817 - phosphate ion transport GO:2000142 - regulation of DNA-templated transcription, initiation

Note(s):

Note(s): ...[more].

Evidence:

[COMP-AINF-FN-FROM-SEQ] Automated inference of function from sequence
[EXP-IDA-PURIFIED-PROTEIN] Assay of protein purified to homogeneity
[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

Reference(s):

[1] Heyde M., et al., 1983
[2] Kim SK., et al., 2000
[3] Makino K., et al., 1988
[4] Makino K., et al., 1986
[5] Pao GM., et al., 1995
[6] Parkinson JS. 1993
[7] Parkinson JS., et al., 1992
[8] Pratt C., et al., 1977
[9] Saier MH. 1994
[10] Shinagawa H., et al., 1983
[11] Stock JB., et al., 1990
[12] Zuckier G., et al., 1980

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

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PRIDE:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMART:

SMR:

SWISSMODEL:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	PhoB
Repressed by:	AlpA

Reference(s)
[1] Heyde M., Portalier R., 1983, Isolation and characterization of a new type of Escherichia coli K12 phoB mutants., Mol Gen Genet 190(1):122-7
[2] Kim SK., Kimura S., Shinagawa H., Nakata A., Lee KS., Wanner BL., Makino K., 2000, Dual transcriptional regulation of the Escherichia coli phosphate-starvation-inducible psiE gene of the phosphate regulon by PhoB and the cyclic AMP (cAMP)-cAMP receptor protein complex., J Bacteriol 182(19):5596-9
[3] Makino K., Shinagawa H., Amemura M., Kimura S., Nakata A., Ishihama A., 1988, Regulation of the phosphate regulon of Escherichia coli. Activation of pstS transcription by PhoB protein in vitro., J Mol Biol 203(1):85-95
[4] Makino K., Shinagawa H., Amemura M., Nakata A., 1986, Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12., J Mol Biol 190(1):37-44
[5] Pao GM., Saier MH., 1995, Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution., J Mol Evol 40(2):136-54
[6] Parkinson JS., 1993, Signal transduction schemes of bacteria., Cell 73(5):857-71
[7] Parkinson JS., Kofoid EC., 1992, Communication modules in bacterial signaling proteins., Annu Rev Genet 26:71-112
[8] Pratt C., Torriani A., 1977, Complementation test between alkaline phosphatase regulatory mutations phoB and phoRc in Escherichia coli., Genetics 85(2):203-8
[9] Saier MH., 1994, Bacterial sensor kinase/response regulator systems: an introduction., Res Microbiol 145(5-6):349-55
[10] Shinagawa H., Makino K., Nakata A., 1983, Regulation of the pho regulon in Escherichia coli K-12. Genetic and physiological regulation of the positive regulatory gene phoB., J Mol Biol 168(3):477-88
[11] Stock JB., Stock AM., Mottonen JM., 1990, Signal transduction in bacteria., Nature 344(6265):395-400
[12] Zuckier G., Ingenito E., Torriani A., 1980, Pleiotropic effects of alkaline phosphatase regulatory mutations phoB and phoT on anaerobic growth of and polyphosphate synthesis in Escherichia coli., J Bacteriol 143(2):934-41
[13] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78