RegulonDB

Gene
Name:	priC
Synonym(s):	ECK0461, EG10765, b0467
Genome position(nucleotides):	490285 <-- 490812 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	58.9
External database links:

ASAP:	ABE-0001619
CGSC:	29161
ECHOBASE:	EB0758
OU-MICROARRAY:	b0467
PortEco:	priC
STRING:	511145.b0467
COLOMBOS:	priC

Product

Name:

primosomal replication protein N''

Synonym(s):

PriC

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

20.375

Isoelectric point:

10.613

Motif(s):

Type	Positions	Sequence
	2 -> 175	KTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
	9 -> 173	KLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARL

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.1 - DNA related --> 2.1.1 - DNA replication
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:1990077 - primosome complex
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding
	biological_process	GO:0006261 - DNA-dependent DNA replication GO:0006270 - DNA replication initiation GO:0006269 - DNA replication, synthesis of RNA primer

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity

Reference(s):

[1] Ng JY., et al., 1996
[2] Zavitz KH., et al., 1991

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

priC-ybaM

Operon arrangement:

Transcription unit	Promoter

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	ybaMp3	490324	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 5.266. P-value: 2.000e-5 Read more >	[ICWHO]	[3]
promoter	ybaNp1	490718	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -2.53. P-value: 2.593e-5 Read more >	[ICWHO]	[3]
promoter	ybaNp8	490857	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.023. P-value: 6.507e-5 Read more >	[ICWHO]	[3]
promoter	ybaNp6	490873	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.77. P-value: 8.356e-5 Read more >	[ICWHO]	[3]
promoter	ybaNp9	490879	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 11.269. P-value: 2.289e-7 Read more >	[ICWHO]	[3]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Ng JY., Marians KJ., 1996, The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes., J Biol Chem 271(26):15642-8
[2] Zavitz KH., DiGate RJ., Marians KJ., 1991, The priB and priC replication proteins of Escherichia coli. Genes, DNA sequence, overexpression, and purification., J Biol Chem 266(21):13988-95
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78