RegulonDB

Gene
Name:	prs
Synonym(s):	ECK1195, EG10774, b1207, dnaR, prsA
Genome position(nucleotides):	1260928 <-- 1261875
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.53
External database links:

ASAP:	ABE-0004054
CGSC:	357
ECHOBASE:	EB0767
ECOLIHUB:	prs
ECOO157CYC:	PRSA
OU-MICROARRAY:	b1207
STRING:	511145.b1207
COLOMBOS:	prs

Gene

Name:

prs

Synonym(s):

ECK1195, EG10774, b1207, dnaR, prsA

Genome position(nucleotides):

1260928 <-- 1261875

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

52.53

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

ECOO157CYC:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

Comment

2 -> 315

PDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH

UniProt: Ribose-phosphate pyrophosphokinase.

4 -> 121

MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVG

37 -> 39

DGE

UniProt: ATP.

96 -> 97

UniProt: ATP.

127 -> 127

UniProt: In Ref. 1; AAA24431..

Type

Positions

Sequence

Comment

2 -> 315

UniProt: Ribose-phosphate pyrophosphokinase.

4 -> 121

MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVG

37 -> 39

DGE

UniProt: ATP.

96 -> 97

UniProt: ATP.

127 -> 127

UniProt: In Ref. 1; AAA24431..

129 -> 129

UniProt: In mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP..

204 -> 313

VMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMF

220 -> 220

UniProt: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type..

221 -> 221

UniProt: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions..

224 -> 224

UniProt: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type..

Multifun Terms (GenProtEC)

1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.3 - purine ribonucleotide biosynthesis

Gene Ontology Terms (GO)

cellular_component

GO:0002189 - ribose phosphate diphosphokinase complex
GO:0005737 - cytoplasm
GO:0005829 - cytosol

molecular_function

GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004749 - ribose phosphate diphosphokinase activity
GO:0000287 - magnesium ion binding
GO:0042802 - identical protein binding
GO:0043531 - ADP binding
GO:0042301 - phosphate ion binding

biological_process

GO:0008152 - metabolic process
GO:0006164 - purine nucleotide biosynthetic process
GO:0009165 - nucleotide biosynthetic process
GO:0044249 - cellular biosynthetic process
GO:0016310 - phosphorylation
GO:0009156 - ribonucleoside monophosphate biosynthetic process
GO:0006015 - 5-phosphoribose 1-diphosphate biosynthetic process
GO:0034214 - protein hexamerization

Transcriptional Regulation
	Display Regulation
Repressed by:	PurR

Transcriptional Regulation

Display Regulation

Repressed by:

PurR

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1537	1259525	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_1538	1260982	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_1539	1261162	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_1540	1261821	reverse	nd	[RS-EPT-CBR]	[1]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_1537

1259525

reverse

[RS-EPT-CBR]

[1]

promoter

TSS_1538

1260982

reverse

[RS-EPT-CBR]

[1]

promoter

TSS_1539

1261162

reverse

[RS-EPT-CBR]

[1]

promoter

TSS_1540

1261821

reverse

[RS-EPT-CBR]

[1]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.