RegulonDB RegulonDB 10.9: Gene Form
   

psd gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mscM rsgA psd CpxR TSS_5002 TSS_5002 psdp psdp TSS_5001 TSS_5001 TSS_5000 TSS_5000 TSS_4999 TSS_4999

Gene      
Name: psd    Texpresso search in the literature
Synonym(s): ECK4156, EG10775, b4160
Genome position(nucleotides): 4389392 <-- 4390360 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.46
External database links:  
ASAP:
ABE-0013623
CGSC:
356
ECHOBASE:
EB0768
ECOLIHUB:
psd
OU-MICROARRAY:
b4160
STRING:
511145.b4160
COLOMBOS: psd


Product      
Name: phosphatidylserine decarboxylase proenzyme
Synonym(s): Psd, phosphatidylserine decarboxylase, pi; subunit
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 35.934
Isoelectric point: 5.448
Motif(s):
 
Type Positions Sequence
1 -> 253 MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKYYKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEEDKILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYVPGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTITPPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLG
254 -> 254 S
63 -> 282 FNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEEDKILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYVPGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTITPPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSVTKIGQP

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.1 - phospholipid
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
molecular_function GO:0016829 - lyase activity
GO:0016831 - carboxy-lyase activity
GO:0004609 - phosphatidylserine decarboxylase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0006629 - lipid metabolic process
GO:0008654 - phospholipid biosynthetic process
GO:0016540 - protein autoprocessing
GO:0006646 - phosphatidylethanolamine biosynthetic process
GO:0031638 - zymogen activation
Note(s): Note(s): ...[more].
Reference(s): [1] Bell RM., et al., 1971
[2] Chamberlain BK., et al., 1978
[3] Denkins YM., et al., 1986
[4] Dowhan W., et al., 1992
[5] Langley KE., et al., 1982
[6] Ohta T., et al., 1977
[7] Rizzolo LJ. 1981
[8] Tyhach RJ., et al., 1979
[9] White DA., et al., 1971
External database links:  
ECOCYC:
PSD-MONOMER
ECOLIWIKI:
b4160
INTERPRO:
IPR033178
INTERPRO:
IPR033177
INTERPRO:
IPR003817
PANTHER:
PTHR10067
PDB:
6L06
PFAM:
PF02666
PRIDE:
P0A8K1
REFSEQ:
NP_418584
UNIPROT:
P0A8K1


Operon      
Name: psd-mscM         
Operon arrangement:
Transcription unit        Promoter
psd-mscM


Transcriptional Regulation      
Display Regulation             
Repressed by: CpxR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4999 4386346 reverse nd [RS-EPT-CBR] [10]
  promoter TSS_5000 4386996 reverse nd [RS-EPT-CBR] [10]
  promoter TSS_5001 4389996 reverse nd [RS-EPT-CBR] [10]
  promoter TSS_5002 4390663 reverse nd [RS-EPT-CBR] [10]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Bell RM., Mavis RD., Osborn MJ., Vagelos PR., 1971, Enzymes of phospholipid metabolism: localization in the cytoplasmic and outer membrane of the cell envelope of Escherichia coli and Salmonella typhimurium., Biochim Biophys Acta 249(2):628-35

 [2] Chamberlain BK., Webster RE., 1978, Effect of membrane-associated f1 bacteriophage coat protein upon the activity of Escherichia coli phosphatidylserine synthetase., J Bacteriol 135(3):883-7

 [3] Denkins YM., Schroit AJ., 1986, Phosphatidylserine decarboxylase: generation of asymmetric vesicles and determination of the transbilayer distribution of fluorescent phosphatidylserine in model membrane systems., Biochim Biophys Acta 862(2):343-51

 [4] Dowhan W., Li QX., 1992, Phosphatidylserine decarboxylase from Escherichia coli., Methods Enzymol 209:348-59

 [5] Langley KE., Hawrot E., Kennedy EP., 1982, Membrane assembly: movement of phosphatidylserine between the cytoplasmic and outer membranes of Escherichia coli., J Bacteriol 152(3):1033-41

 [6] Ohta T., Okuda S., Takahashi H., 1977, Relationship between phospholipid compositions and transport activities of amino acids in Escherichia coli membrane vesicles., Biochim Biophys Acta 466(1):44-56

 [7] Rizzolo LJ., 1981, Kinetics and protein subunit interactions of Escherichia coli phosphatidylserine decarboxylase in detergent solution., Biochemistry 20(4):868-73

 [8] Tyhach RJ., Hawrot E., Satre M., Kennedy EP., 1979, Increased synthesis of phosphatidylserine decarboxylase in a strain of Escherichia coli bearing a hybrid plasmid. Altered association of enzyme with the membrane., J Biol Chem 254(3):627-33

 [9] White DA., Albright FR., Lennarz WJ., Schnaitman CA., 1971, Distribution of phospholipid-synthesizing enzymes in the wall and membrane subfractions of the envelope of Escherichia coli., Biochim Biophys Acta 249(2):636-42

 [10] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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