RegulonDB RegulonDB 11.1: Gene Form
   

pstB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pstA pstB phoU LeuO terminator pstBp2 pstBp2 pstBp1 pstBp1 pstBp3 pstBp3 phoUp phoUp phoUp2 phoUp2

Gene      
Name: pstB    Texpresso search in the literature
Synonym(s): ECK3718, EG10783, b3725, phoT
Genome position(nucleotides): 3907593 <-- 3908366
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.33
External database links:  
ASAP:
 ABE-0012179
CGSC:
 18004
ECHOBASE:
 EB0776
ECOLIHUB:
 pstB
OU-MICROARRAY:
 b3725
STRING:
 511145.b3725
COLOMBOS: pstB


Product      
Name: phosphate ABC transporter ATP binding subunit
Synonym(s): PhoT, PstB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 29.027
Isoelectric point: 6.517
Motif(s):
 
Type Positions Sequence Comment
2 -> 257 SMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGPSGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTPFPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQRLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARCSDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG UniProt: Phosphate import ATP-binding protein PstB.
11 -> 252 IQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGPSGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTPFPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQRLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARCSDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYI UniProt: ABC transporter.
26 -> 181 LKNINLDIAKNQVTAFIGPSGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTPFPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQRLCIARGIAIRPEVLLLDEPC
43 -> 50 GPSGCGKS UniProt: ATP.
48 -> 48 G UniProt: Loss of phosphate transport..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.1 - phosphorous metabolism
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.a - ABC superfamily ATP binding cytoplasmic component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0043190 - ATP-binding cassette (ABC) transporter complex
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
molecular_function GO:0005515 - protein binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0015415 - ATPase-coupled phosphate ion transmembrane transporter activity
GO:0005315 - inorganic phosphate transmembrane transporter activity
biological_process GO:0010921 - regulation of phosphatase activity
GO:0006817 - phosphate ion transport
GO:0035435 - phosphate ion transmembrane transport
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0AAH0
DIP:
 DIP-47851N
ECOCYC:
 PSTB-MONOMER
ECOLIWIKI:
 b3725
INTERPRO:
 IPR005670
INTERPRO:
 IPR027417
INTERPRO:
 IPR015850
INTERPRO:
 IPR017871
INTERPRO:
 IPR003593
INTERPRO:
 IPR003439
PANTHER:
 PTHR43423
PFAM:
 PF00005
PRIDE:
 P0AAH0
PRODB:
 PRO_000023622
PROSITE:
 PS51238
PROSITE:
 PS50893
PROSITE:
 PS00211
REFSEQ:
 NP_418181
SMART:
 SM00382
SMR:
 P0AAH0
UNIPROT:
 P0AAH0


Operon      
Name: pstSCAB-phoU         
Operon arrangement:
Transcription unit        Promoter
phoU
pstB-phoU
pstB-phoU
pstB-phoU
pstCAB-phoU
pstCAB-phoU
pstS
pstSCA
pstSCAB-phoU
pstS
pstSCA
pstSCAB-phoU


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, IHF, PhoB


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter phoUp2 3907646 reverse nd [COMP-AINF] [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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