RegulonDB RegulonDB 11.1: Gene Form
   

pyrB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pyrB pyrI pyrL unknown Lrp anti-anti-terminator anti-terminator TSS_5094 TSS_5094 TSS_5093 TSS_5093 TSS_5092 (cluster) TSS_5092 (cluster) TSS_5091 TSS_5091 TSS_5090 (cluster) TSS_5090 (cluster) TSS_5089 TSS_5089

Gene      
Name: pyrB    Texpresso search in the literature
Synonym(s): ECK4240, EG10805, b4245
Genome position(nucleotides): 4471460 <-- 4472395
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.74
External database links:  
ASAP:
 ABE-0013892
CGSC:
 330
ECHOBASE:
 EB0798
ECOLIHUB:
 pyrB
OU-MICROARRAY:
 b4245
STRING:
 511145.b4245
COLOMBOS: pyrB


Product      
Name: aspartate carbamoyltransferase catalytic subunit
Synonym(s): PyrB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 34.427
Isoelectric point: 6.59
Motif(s):
 
Type Positions Sequence Comment
2 -> 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL UniProt: Aspartate carbamoyltransferase catalytic subunit.
8 -> 148 KHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQE
61 -> 61 E UniProt: In Ref. 1; AAA24474 and 6; AA sequence..
87 -> 87 E UniProt: In Ref. 6; AA sequence..
91 -> 91 D UniProt: In Ref. 6; AA sequence..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.4 - pyrimidine ribonucleotide biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0009347 - aspartate carbamoyltransferase complex
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0016597 - amino acid binding
GO:0004070 - aspartate carbamoyltransferase activity
GO:0016743 - carboxyl- or carbamoyltransferase activity
GO:0042802 - identical protein binding
biological_process GO:0006221 - pyrimidine nucleotide biosynthetic process
GO:0006520 - cellular amino acid metabolic process
GO:0006807 - nitrogen compound metabolic process
GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic process
GO:0044205 - 'de novo' UMP biosynthetic process
GO:0070207 - protein homotrimerization
External database links:  
ALPHAFOLD:
 P0A786
DIP:
 DIP-35089N
ECOCYC:
 ASPCARBCAT-MONOMER
ECOLIWIKI:
 b4245
INTERPRO:
 IPR006132
INTERPRO:
 IPR036901
INTERPRO:
 IPR002082
INTERPRO:
 IPR006130
INTERPRO:
 IPR006131
MINT:
 P0A786
PANTHER:
 PTHR11405:SF16
PDB:
 1ACM
PDB:
 1AT1
PDB:
 1D09
PDB:
 1EKX
PDB:
 1EZZ
PDB:
 1F1B
PDB:
 1I5O
PDB:
 1NBE
PDB:
 1Q95
PDB:
 1R0B
PDB:
 1R0C
PDB:
 1RAA
PDB:
 1RAB
PDB:
 1RAC
PDB:
 1RAD
PDB:
 1RAE
PDB:
 1RAF
PDB:
 1RAG
PDB:
 1RAH
PDB:
 1RAI
PDB:
 1SKU
PDB:
 1TTH
PDB:
 1TU0
PDB:
 1TUG
PDB:
 1XJW
PDB:
 1ZA1
PDB:
 1ZA2
PDB:
 2A0F
PDB:
 2AIR
PDB:
 2AT1
PDB:
 2ATC
PDB:
 2FZC
PDB:
 2FZG
PDB:
 2FZK
PDB:
 2H3E
PDB:
 2HSE
PDB:
 2IPO
PDB:
 2QG9
PDB:
 2QGF
PDB:
 3AT1
PDB:
 3CSU
PDB:
 3D7S
PDB:
 3MPU
PDB:
 3NPM
PDB:
 4AT1
PDB:
 4E2F
PDB:
 4F04
PDB:
 4FYV
PDB:
 4FYW
PDB:
 4FYX
PDB:
 4FYY
PDB:
 5AT1
PDB:
 5VMQ
PDB:
 6AT1
PDB:
 6KJ7
PDB:
 6KJ8
PDB:
 6KJ9
PDB:
 6KJA
PDB:
 6KJB
PDB:
 7AT1
PDB:
 8AT1
PDB:
 8ATC
PDB:
 9ATC
PFAM:
 PF00185
PFAM:
 PF02729
PRIDE:
 P0A786
PRINTS:
 PR00100
PRODB:
 PRO_000023657
PROSITE:
 PS00097
REFSEQ:
 NP_418666
SMR:
 P0A786
UNIPROT:
 P0A786


Operon      
Name: pyrLBI         
Operon arrangement:
Transcription unit        Promoter
pyrLBI
pyrLBI


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_5089 4471479 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_5090 (cluster) 4471486 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_5091 4471513 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_5092 (cluster) 4471516 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_5093 4471843 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_5094 4472421 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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