RegulonDB RegulonDB 10.9: Gene Form
   

pyrC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pyrC yceB dinI Fur PurR NagC LexA Cis-reg; leader anti-anti-terminator anti-terminator terminator TSS_1335 TSS_1335 pyrCp pyrCp dinIp dinIp

Gene      
Name: pyrC    Texpresso search in the literature
Synonym(s): ECK1047, EG10806, b1062
Genome position(nucleotides): 1121561 <-- 1122607 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.1
External database links:  
ASAP:
ABE-0003607
CGSC:
329
ECHOBASE:
EB0799
ECOLIHUB:
pyrC
OU-MICROARRAY:
b1062
STRING:
511145.b1062
COLOMBOS: pyrC


Product      
Name: dihydroorotase
Synonym(s): PyrC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 38.827
Isoelectric point: 6.148
Motif(s):
 
Type Positions Sequence
21 -> 21 R
255 -> 255 H
2 -> 348 TAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMPNLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAAKLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIESVMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRPHLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTALGSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLVPFLAGETVRWSVKQ
15 -> 307 DWHLHLRDGDMLKTVVPYTSEIYGRAIVMPNLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAAKLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIESVMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRPHLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTALGSYATVFEEMNALQHFEAFCSVNGPQFYGLP
19 -> 21 HLR

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.2 - pyrimidine biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016787 - hydrolase activity
GO:0046872 - metal ion binding
GO:0008270 - zinc ion binding
GO:0016812 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
GO:0004151 - dihydroorotase activity
biological_process GO:0006221 - pyrimidine nucleotide biosynthetic process
GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic process
GO:0019856 - pyrimidine nucleobase biosynthetic process
GO:0044205 - 'de novo' UMP biosynthetic process
External database links:  
DIP:
DIP-10624N
ECOCYC:
DIHYDROOROT-MONOMER
ECOLIWIKI:
b1062
INTERPRO:
IPR006680
INTERPRO:
IPR004721
INTERPRO:
IPR002195
INTERPRO:
IPR032466
MODBASE:
P05020
PANTHER:
PTHR43137
PDB:
2Z28
PDB:
2Z29
PDB:
2Z2A
PDB:
2Z2B
PDB:
6HG1
PDB:
2Z27
PDB:
2Z26
PDB:
2Z25
PDB:
2Z24
PDB:
2EG8
PDB:
2EG7
PDB:
2EG6
PDB:
2E25
PDB:
1XGE
PDB:
1J79
PFAM:
PF01979
PRIDE:
P05020
PRODB:
PRO_000023658
PROSITE:
PS00482
PROSITE:
PS00483
REFSEQ:
NP_415580
SMR:
P05020
UNIPROT:
P05020


Operon      
Name: pyrC         
Operon arrangement:
Transcription unit        Promoter
pyrC


Transcriptional Regulation      
Display Regulation             
Repressed by: PurR, Fur


RNA cis-regulatory element    
Attenuation: Transcriptional
   
Cis-reg; leader PyrC leader
   
   


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1335 1123108 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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