RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

thyA gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	thyA
Synonym(s):	ECK2823, EG11002, b2827
Genome position(nucleotides):	2964361 <-- 2965155 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.07
External database links:

ASAP:	ABE-0009268
CGSC:	104
ECHOBASE:	EB0995
NCBI-GENE:	949035
OU-MICROARRAY:	b2827
PortEco:	thyA
STRING:	511145.b2827
COLOMBOS:	thyA

Product

Name:

thymidylate synthase

Synonym(s):

ThyA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

30.48

Isoelectric point:

5.931

Motif(s):

Type	Positions	Sequence
	207 -> 209	HLY
	50 -> 50	C
	177 -> 177	N
	166 -> 169	RSCD
	2 -> 264	KQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLVTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.17 - formyl-THF biosynthesis
		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0008168 - methyltransferase activity GO:0016740 - transferase activity GO:0003723 - RNA binding GO:0004799 - thymidylate synthase activity GO:0000287 - magnesium ion binding GO:0042803 - protein homodimerization activity
	biological_process	GO:0006417 - regulation of translation GO:0009165 - nucleotide biosynthetic process GO:0009314 - response to radiation GO:0032259 - methylation GO:0006231 - dTMP biosynthetic process GO:0006235 - dTTP biosynthetic process

External database links:

DIP:

DIP-48261N

ECOCYC:

THYMIDYLATESYN-MONOMER

ECOLIWIKI:

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Operon

Name:

lgt-thyA

Operon arrangement:

Transcription unit	Promoter
thyA	thyAp
lgt	lgtp
lgt-thyA	lgtp

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_3128 (cluster)	2965245		reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2965244 to 2965245, where the highest frequency is at position 2963267. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[1]
	promoter	thyAp2	2965348		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 6.735. P-value: 3.136e-5 Read more >	[ICWHO]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RegulonDB