RegulonDB RegulonDB 10.8: Gene Form
   

tnaA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tnaA tnaB tnaC terminator

Gene      
Name: tnaA    Texpresso search in the literature
Synonym(s): ECK3701, EG11005, b3708, ind, tnaR
Genome position(nucleotides): 3888730 --> 3890145 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
50.85
External database links:  
ASAP:
ABE-0012133
CGSC:
101
ECHOBASE:
EB0998
NCBI-GENE:
948221
OU-MICROARRAY:
b3708
PortEco:
tnaA
STRING:
511145.b3708
COLOMBOS: tnaA


Product      
Name: tryptophanase
Synonym(s): Ind, TnaA, TnaR
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 52.773
Isoelectric point: 6.135
Motif(s):
 
Type Positions Sequence
298 -> 298 C
379 -> 380 QA
49 -> 437 DLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIE
137 -> 140 DTTQ
294 -> 294 C

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0032991 - protein-containing complex
GO:0060187 - cell pole
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0016830 - carbon-carbon lyase activity
GO:0030170 - pyridoxal phosphate binding
GO:0009034 - tryptophanase activity
GO:0030955 - potassium ion binding
GO:0042802 - identical protein binding
GO:0080146 - L-cysteine desulfhydrase activity
biological_process GO:0006568 - tryptophan metabolic process
GO:0006520 - cellular amino acid metabolic process
GO:0009072 - aromatic amino acid family metabolic process
GO:0006569 - tryptophan catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Chong H., et al., 2014
[2] Gaimster H., et al., 2015
[3] Geng H., et al., 2015
[4] Taylor HV., et al., 1978
External database links:  
DIP:
DIP-31878N
ECOCYC:
TRYPTOPHAN-MONOMER
ECOLIWIKI:
b3708
INTERPRO:
IPR015421
INTERPRO:
IPR015424
INTERPRO:
IPR001597
INTERPRO:
IPR011166
INTERPRO:
IPR013440
INTERPRO:
IPR015422
INTERPRO:
IPR018176
MINT:
MINT-1267429
MODBASE:
P0A853
PDB:
5D8G
PDB:
4W1Y
PDB:
4UP2
PDB:
2V1P
PDB:
2V0Y
PDB:
2OQX
PDB:
2C44
PDB:
4W4H
PFAM:
PF01212
PRIDE:
P0A853
PRODB:
PRO_000024075
PROSITE:
PS00853
REFSEQ:
NP_418164
SMR:
P0A853
SWISSMODEL:
P0A853
UNIPROT:
P0A853


Operon      
Name: tnaCAB         
Operon arrangement:
Transcription unit        Promoter
tnaC
tnaCAB


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, TorR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Chong H., Geng H., Zhang H., Song H., Huang L., Jiang R., 2014, Enhancing E. coli isobutanol tolerance through engineering its global transcription factor cAMP receptor protein (CRP)., Biotechnol Bioeng 111(4):700-8

 [2] Gaimster H., Summers D., 2015, Regulation of Indole Signalling during the Transition of E. coli from Exponential to Stationary Phase., PLoS One 10(9):e0136691

 [3] Geng H., Jiang R., 2015, cAMP receptor protein (CRP)-mediated resistance/tolerance in bacteria: mechanism and utilization in biotechnology., Appl Microbiol Biotechnol 99(11):4533-43

 [4] Taylor HV., Yudkin MD., 1978, Synthesis of tryptophanase in Escherichia coli: isolation and characterization of a structural-gene mutant and two regulatory mutants., Mol Gen Genet 165(1):95-102


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