RegulonDB RegulonDB 11.1: Gene Form
   

trpB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

trpC trpB trpA yciG H-NS McbR H-NS McbR TSS_1694 TSS_1694 TSS_1693 TSS_1693 TSS_1692 TSS_1692 TSS_1691 TSS_1691 TSS_1690 (cluster) TSS_1690 (cluster) TSS_1689 TSS_1689 TSS_1688 TSS_1688 TSS_1687 TSS_1687 trpAp7 trpAp7 trpAp5 trpAp5 trpAp4 trpAp4 trpAp8 trpAp8 yciGp yciGp

Gene      
Name: trpB    Texpresso search in the literature
Synonym(s): ECK1255, EG11025, b1261
Genome position(nucleotides): 1317222 <-- 1318415
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.69
External database links:  
ASAP:
 ABE-0004234
CGSC:
 73
ECHOBASE:
 EB1018
ECOLIHUB:
 trpB
OU-MICROARRAY:
 b1261
STRING:
 511145.b1261
COLOMBOS: trpB


Product      
Name: tryptophan synthase subunit β
Synonym(s): beta; subunit, TrpB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 42.983
Isoelectric point: 6.02
Motif(s):
 
Type Positions Sequence Comment
2 -> 397 TTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQFNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILEREGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALEAFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILKARGEI UniProt: Tryptophan synthase beta chain.
52 -> 375 YAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILEREGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALEAFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVN
78 -> 78 E UniProt: In Ref. 7; AA sequence..
281 -> 281 G UniProt: In mutant TRPB8..
365 -> 365 N UniProt: In Ref. 10; AA sequence..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.15 - tryptophan
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016829 - lyase activity
GO:0030170 - pyridoxal phosphate binding
GO:0004834 - tryptophan synthase activity
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
biological_process GO:0000162 - tryptophan biosynthetic process
GO:0006568 - tryptophan metabolic process
GO:0008652 - cellular amino acid biosynthetic process
GO:0009073 - aromatic amino acid family biosynthetic process
External database links:  
ALPHAFOLD:
 P0A879
DIP:
 DIP-1036N
ECOCYC:
 TRYPSYN-BPROTEIN
ECOLIWIKI:
 b1261
INTERPRO:
 IPR023026
INTERPRO:
 IPR036052
INTERPRO:
 IPR006654
INTERPRO:
 IPR006653
INTERPRO:
 IPR001926
MINT:
 P0A879
MODBASE:
 P0A879
PANTHER:
 PTHR42882
PDB:
 2DH6
PDB:
 2DH5
PFAM:
 PF00291
PRIDE:
 P0A879
PRODB:
 PRO_000024118
PROSITE:
 PS00168
REFSEQ:
 NP_415777
SMR:
 P0A879
SWISSMODEL:
 P0A879
UNIPROT:
 P0A879


Operon      
Name: trpLEDCBA         
Operon arrangement:
Transcription unit        Promoter
trpCBA
trpL
trpLEDCBA


Transcriptional Regulation      
Display Regulation             
Repressed by: TrpR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter trpAp8 1317241 reverse nd [COMP-AINF] [1]
  promoter trpAp4 1317303 reverse nd [COMP-AINF] [1]
  promoter trpAp5 1317306 reverse nd [COMP-AINF] [1]
  promoter trpAp7 1317339 reverse nd [COMP-AINF] [1]
  promoter TSS_1687 1317876 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1688 1317878 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1689 1318430 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1690 (cluster) 1318512 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1691 1318516 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1692 1318535 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1693 1318537 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1694 1318607 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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