RegulonDB

RegulonDB RegulonDB 11.1: Gene Form

trxA gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	trxA
Synonym(s):	ECK3773, EG11031, b3781, dasC, fipA, tsnC
Genome position(nucleotides):	3965761 --> 3966090
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.91
Reference(s):	[1] Lim CJ., et al., 1985
External database links:

ASAP:	ABE-0012357
CGSC:	65
ECHOBASE:	EB1024
ECOLIHUB:	trxA
MIM:	616811
OU-MICROARRAY:	b3781
STRING:	511145.b3781
COLOMBOS:	trxA

Product

Name:

thioredoxin 1

Synonym(s):

DasC, FipA, TRX1, TrxA, TsnC, thioredoxin(SH)₂

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol,inner membrane

Molecular weight:

11.807

Isoelectric point:

4.457

Motif(s):

Type	Positions	Sequence	Comment
	2 -> 109	SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA	UniProt: Thioredoxin.
	5 -> 105	IIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
	72 -> 73	GI	UniProt: In Ref. 8; AA sequence..
	88 -> 88	A	UniProt: In Ref. 5; AAA24696..

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.15 - large molecule carriers --> 1.6.15.2 - thioredoxin, glutaredoxin
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
	molecular_function	GO:0005515 - protein binding GO:0016491 - oxidoreductase activity GO:0015035 - protein-disulfide reductase activity GO:0030337 - DNA polymerase processivity factor activity
	biological_process	GO:1900264 - positive regulation of DNA-directed DNA polymerase activity GO:0045454 - cell redox homeostasis

Note(s):

Note(s): ...[more].

Evidence:

[IDA-PURIFIED-PROTEIN-NH] Assay of protein purified to homogeneity from its native host

Reference(s):

[2] Gleason FK., et al., 1990
[3] Jacob C., et al., 2011

External database links:

ALPHAFOLD:

P0AA25

DIP:

DIP-31856N

ECOCYC:

RED-THIOREDOXIN-MONOMER

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Operon

Name:

trxA

Operon arrangement:

Transcription unit	Promoter
trxA	trxAp1
trxA	trxAp3
trxA	trxAp2

Transcriptional Regulation
	Display Regulation
Repressed by:	CRP

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_4410	3964499	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4411	3964980	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4412	3965171	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4413	3965222	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4414 (cluster)	3965617	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4415	3965630	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4417	3965652	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4418	3965691	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4419 (cluster)	3965693	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4420	3965697	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4421	3965740	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4422	3965743	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4423	3965796	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4425 (cluster)	3966246	forward	nd	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Lim CJ., Geraghty D., Fuchs JA., 1985, Cloning and nucleotide sequence of the trxA gene of Escherichia coli K-12., J Bacteriol 163(1):311-6
[2] Gleason FK., Lim CJ., Gerami-Nejad M., Fuchs JA., 1990, Characterization of Escherichia coli thioredoxins with altered active site residues., Biochemistry 29(15):3701-9
[3] Jacob C., Kriznik A., Boschi-Muller S., Branlant G., 2011, Thioredoxin 2 from Escherichia coli is not involved in vivo in the recycling process of methionine sulfoxide reductase activities., FEBS Lett 585(12):1905-9
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB