RegulonDB RegulonDB 11.1: Gene Form
   

tufB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tufB secE thrT OxyS terminator anti-terminator anti-anti-terminator TSS_4736 TSS_4736 TSS_4735 TSS_4735 TSS_4734 (cluster) TSS_4734 (cluster) TSS_4733 (cluster) TSS_4733 (cluster) TSS_4732 (cluster) TSS_4732 (cluster) TSS_4731 (cluster) TSS_4731 (cluster) TSS_4730 TSS_4730 TSS_4729 (cluster) TSS_4729 (cluster) TSS_4728 TSS_4728 secEp secEp TSS_4727 TSS_4727 TSS_4726 TSS_4726 TSS_4725 TSS_4725 TSS_4724 TSS_4724 TSS_4723 (cluster) TSS_4723 (cluster) TSS_4722 (cluster) TSS_4722 (cluster) TSS_4721 TSS_4721 TSS_4720 (cluster) TSS_4720 (cluster) TSS_4719 TSS_4719 TSS_4718 (cluster) TSS_4718 (cluster) TSS_4717 TSS_4717 TSS_4716 TSS_4716 TSS_4715 TSS_4715 TSS_4714 TSS_4714 TSS_4713 TSS_4713 TSS_4712 TSS_4712 TSS_4711 TSS_4711 TSS_4710 TSS_4710 TSS_4709 (cluster) TSS_4709 (cluster) TSS_4708 TSS_4708 TSS_4707 TSS_4707 TSS_4706 TSS_4706 TSS_4705 TSS_4705 TSS_4704 TSS_4704 TSS_4703 TSS_4703 TSS_4702 (cluster) TSS_4702 (cluster)

Gene      
Name: tufB    Texpresso search in the literature
Synonym(s): ECK3971, EG11037, b3980, kirT, pulT
Genome position(nucleotides): 4175944 --> 4177128
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.42
External database links:  
ASAP:
 ABE-0013020
CGSC:
 60
ECHOBASE:
 EB1030
ECOLIHUB:
 tufB
ECOO157CYC:
 TUFB
MIM:
 610678
OU-MICROARRAY:
 b3980
STRING:
 511145.b3980
COLOMBOS: tufB


Product      
Name: translation elongation factor Tu 2
Synonym(s): EF-Tu, KirT, PulT, TufB, elongation factor Tu
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cytosol
Molecular weight: 43.314
Isoelectric point: 5.13
Motif(s):
 
Type Positions Sequence Comment
2 -> 394 SKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS UniProt: Elongation factor Tu 2.
10 -> 204 KPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPE UniProt: tr-type G.
19 -> 26 GHVDHGKT UniProt: G1; Sequence Annotation Type: region of interest.
20 -> 20 H UniProt: No change in binding GDP and 3-fold reduction in binding EF-Ts..
60 -> 64 GITIN UniProt: G2; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
Gene Ontology Terms (GO)  
cellular_component GO:0032045 - guanyl-nucleotide exchange factor complex
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
molecular_function GO:0003746 - translation elongation factor activity
GO:0005515 - protein binding
GO:0003723 - RNA binding
GO:0000166 - nucleotide binding
GO:0003924 - GTPase activity
GO:0005525 - GTP binding
GO:0097216 - guanosine tetraphosphate binding
biological_process GO:0006412 - translation
GO:0006414 - translational elongation
GO:0046677 - response to antibiotic
Note(s): Note(s): ...[more].
Reference(s): [1] Weijland A., et al., 1992
External database links:  
ALPHAFOLD:
 P0CE48
DIP:
 DIP-60620N
DISPROT:
 DP01965
ECOCYC:
 EG11037-MONOMER
ECOLIWIKI:
 b3980
ECOO157CYC:
 TUFB-MONOMER
INTERPRO:
 IPR009001
INTERPRO:
 IPR009000
INTERPRO:
 IPR005225
INTERPRO:
 IPR004541
INTERPRO:
 IPR004161
INTERPRO:
 IPR004160
INTERPRO:
 IPR041709
INTERPRO:
 IPR000795
INTERPRO:
 IPR027417
INTERPRO:
 IPR031157
INTERPRO:
 IPR033720
MODBASE:
 P02990
PDB:
 4R71
PDB:
 4V6K
PDB:
 4V6L
PDB:
 5AFI
PDB:
 5I4R
PDB:
 5WDT
PDB:
 5WE4
PDB:
 5WE6
PDB:
 5WFK
PDB:
 6EZE
PDB:
 4FWT
PDB:
 3VNV
PDB:
 3VNU
PDB:
 3MMP
PDB:
 3AVY
PDB:
 3AVX
PDB:
 3AVW
PDB:
 3AVV
PDB:
 3AVU
PDB:
 3AVT
PDB:
 3AGQ
PDB:
 3AGP
PDB:
 2BVN
PDB:
 1QZD
PDB:
 1OB2
PDB:
 1LS2
PDB:
 1EFU
PDB:
 1DG1
PDB:
 1EFM
PFAM:
 PF00009
PFAM:
 PF03143
PFAM:
 PF03144
PRIDE:
 P0CE48
PRINTS:
 PR00315
PROSITE:
 PS00301
PROSITE:
 PS51722
REFSEQ:
 NP_418407
SMR:
 P0CE48
UNIPROT:
 P0CE48


Operon      
Name: thrU-tyrU-glyT-thrT-tufB         
Operon arrangement:
Transcription unit        Promoter
thrU-tyrU-glyT-thrT-tufB


Transcriptional Regulation      
Display Regulation             
Activated by: Fis


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4702 (cluster) 4175869 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4703 4175874 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4704 4175883 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4705 4175917 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4706 4175920 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4707 4175926 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4708 4175928 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4709 (cluster) 4175958 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4710 4175968 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4711 4176116 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4712 4176325 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4713 4176332 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4714 4176429 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4715 4176443 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4716 4176445 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4717 4176452 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4718 (cluster) 4176586 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4719 4176591 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4720 (cluster) 4176593 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4721 4176597 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4722 (cluster) 4176659 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4723 (cluster) 4176669 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4724 4176704 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4725 4176727 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4726 4176739 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4727 4176975 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4728 4177213 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4729 (cluster) 4177243 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4730 4177246 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4731 (cluster) 4177278 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4732 (cluster) 4177295 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4733 (cluster) 4177301 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4734 (cluster) 4177329 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4735 4177340 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4736 4177443 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Weijland A., Harmark K., Cool RH., Anborgh PH., Parmeggiani A., 1992, Elongation factor Tu: a molecular switch in protein biosynthesis., Mol Microbiol 6(6):683-8

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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