Gene
Name:	uidA
Synonym(s):	ECK1612, EG11055, b1617, gurA, gusA
Genome position(nucleotides):	1694260 <-- 1696071 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.26
External database links:

ASAP:	ABE-0005410
CGSC:	36
ECHOBASE:	EB1048
ECOLIHUB:	uidA
MIM:	253220
OU-MICROARRAY:	b1617
STRING:	511145.b1617
COLOMBOS:	uidA

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	uidCp5	1692988		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.683. P-value: 9.611e-5 Read more >	[ICWHO]	[14]
	promoter	uidCp6	1693006		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.723. P-value: 1.557e-5 Read more >	[ICWHO]	[14]

Reference(s)
[1] Ahmad S., Hughes MA., Yeh LA., Scott JE., 2012, Potential repurposing of known drugs as potent bacterial β-glucuronidase inhibitors., J Biomol Screen 17(7):957-65
[2] Borisenkov MF., Bakutova LA., Latkin DS., Golovchenko VV., Vityazev FV., 2011, Interaction of microbial β-glucuronidase with vegetable pectins., J Agric Food Chem 59(18):9922-6
[3] Briciu-Burghina C., Heery B., Regan F., 2015, Continuous fluorometric method for measuring β-glucuronidase activity: comparative analysis of three fluorogenic substrates., Analyst 140(17):5953-64
[4] Deckert K., Budiardjo SJ., Brunner LC., Lovell S., Karanicolas J., 2012, Designing allosteric control into enzymes by chemical rescue of structure., J Am Chem Soc 134(24):10055-60
[5] Khan KM., Fakhri MI., Shaikh NN., Saad SM., Hussain S., Perveen S., Choudhary MI., 2014, β-glucuronidase inhibitory studies on coumarin derivatives., Med Chem 10(8):778-82
[6] Kong R., Liu T., Zhu X., Ahmad S., Williams AL., Phan AT., Zhao H., Scott JE., Yeh LA., Wong ST., 2014, Old drug new use--amoxapine and its metabolites as potent bacterial β-glucuronidase inhibitors for alleviating cancer drug toxicity., Clin Cancer Res 20(13):3521-30
[7] LoGuidice A., Wallace BD., Bendel L., Redinbo MR., Boelsterli UA., 2012, Pharmacologic targeting of bacterial β-glucuronidase alleviates nonsteroidal anti-inflammatory drug-induced enteropathy in mice., J Pharmacol Exp Ther 341(2):447-54
[8] Man S., Cheng R., Miao C., Gong Q., Gu Y., Lu X., Han F., Yu W., 2011, Artificial trans-encoded small non-coding RNAs specifically silence the selected gene expression in bacteria., Nucleic Acids Res 39(8):e50
[9] Novel M., Novel G., 1974, [Escherichia coli K 12 mutants, able to grow on methyl-beta-galacturonide: simple constitutive mutants for the synthesis of beta-glucuronidase and double mutants also derepressed for the synthesis of 2 enzymes for glucuronate utilization]., C R Acad Sci Hebd Seances Acad Sci D 279(8):695-8
[10] Novel M., Novel G., 1976, Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: constitutive mutants specifically derepressed for uidA expression., J Bacteriol 127(1):406-17
[11] Novel M., Novel G., 1976, Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: pleiotropic constitutive mutations affecting uxu and uidA expression., J Bacteriol 127(1):418-32
[12] Rasmussen TS., Koldso H., Nakagawa S., Kato A., Schiott B., Jensen HH., 2011, Synthesis of uronic-noeurostegine--a potent bacterial β-glucuronidase inhibitor., Org Biomol Chem 9(22):7807-13
[13] Wilkinson SM., Watson MA., Willis AC., McLeod MD., 2011, Experimental and kinetic studies of the Escherichia coli glucuronylsynthase: an engineered enzyme for the synthesis of glucuronide conjugates., J Org Chem 76(7):1992-2000
[14] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Transcriptional Regulation
	Display Regulation
Activated by:	CRP
Repressed by:	UidR, UxuR