RegulonDB RegulonDB 11.1: Gene Form
   

uvrC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

uvrC uvrY pgsA yecU yecF LexA yecFp yecFp uvrYp2 uvrYp2 TSS_2249 (cluster) TSS_2249 (cluster) uvrCp3 uvrCp3 TSS_2248 TSS_2248 TSS_2247 TSS_2247

Gene      
Name: uvrC    Texpresso search in the literature
Synonym(s): ECK1912, EG11063, b1913
Genome position(nucleotides): 1992874 <-- 1994706
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.72
External database links:  
ASAP:
 ABE-0006370
CGSC:
 19
ECHOBASE:
 EB1056
ECOLIHUB:
 uvrC
OU-MICROARRAY:
 b1913
STRING:
 511145.b1913
COLOMBOS: uvrC


Product      
Name: UvrABC excision nuclease subunit C
Synonym(s): UvrC, excision nuclease subunit C
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 68.188
Isoelectric point: 9.385
Motif(s):
 
Type Positions Sequence Comment
16 -> 94 SQPGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNV UniProt: GIY-YIG.
18 -> 93 PGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYN
42 -> 42 R UniProt: Defective in 3' incision but not in 5' incision..
204 -> 239 DQVLTQLISRMETASQNLEFEEAARIRDQIQAVRRV UniProt: UVR.
205 -> 237 QVLTQLISRMETASQNLEFEEAARIRDQIQAVR

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.2 - DNA
  2 - information transfer --> 2.1 - DNA related --> 2.1.4 - DNA repair
  2 - information transfer --> 2.1 - DNA related --> 2.1.5 - DNA degradation
  5 - cell processes --> 5.6 - protection --> 5.6.1 - radiation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0009380 - excinuclease repair complex
molecular_function GO:0003677 - DNA binding
GO:0004518 - nuclease activity
GO:0009381 - excinuclease ABC activity
biological_process GO:0006281 - DNA repair
GO:0006974 - cellular response to DNA damage stimulus
GO:0009432 - SOS response
GO:0009314 - response to radiation
GO:0006289 - nucleotide-excision repair
GO:0033683 - nucleotide-excision repair, DNA incision
Reference(s): [1] Silva RMB., et al., 2020
[2] Wendel BM., et al., 2021
External database links:  
ALPHAFOLD:
 P0A8G0
DIP:
 DIP-47875N
ECOCYC:
 EG11063-MONOMER
ECOLIWIKI:
 b1913
INTERPRO:
 IPR036876
INTERPRO:
 IPR038476
INTERPRO:
 IPR035901
INTERPRO:
 IPR010994
INTERPRO:
 IPR004791
INTERPRO:
 IPR003583
INTERPRO:
 IPR001943
INTERPRO:
 IPR001162
INTERPRO:
 IPR000305
MODBASE:
 P0A8G0
PDB:
 1KFT
PFAM:
 PF01541
PFAM:
 PF14520
PFAM:
 PF08459
PFAM:
 PF02151
PRIDE:
 P0A8G0
PRODB:
 PRO_000024200
PROSITE:
 PS50164
PROSITE:
 PS50151
PROSITE:
 PS50165
REFSEQ:
 NP_416423
SMART:
 SM00465
SMART:
 SM00278
SMR:
 P0A8G0
SWISSMODEL:
 P0A8G0
UNIPROT:
 P0A8G0


Operon      
Name: uvrYC         
Operon arrangement:
Transcription unit        Promoter
uvrC
uvrYC


Transcriptional Regulation      
Display Regulation             
Repressed by: LexA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2247 1992850 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2248 1994876 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2249 (cluster) 1995403 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Silva RMB., Grodick MA., Barton JK., 2020, UvrC Coordinates an O2 -Sensitive [4Fe4S] Cofactor., J Am Chem Soc 142(25):10964-10977

 [2] Wendel BM., Hollingsworth S., Courcelle CT., Courcelle J., 2021, UV-induced DNA damage disrupts the coordination between replication initiation, elongation and completion., Genes Cells 26(2):94-108

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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