RegulonDB RegulonDB 11.0: Gene Form
   

entH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cstA entA entH CRP ybdDp2 ybdDp2 TSS_727 TSS_727 cstAp3 cstAp3 TSS_726 TSS_726 cstAp2 cstAp2 TSS_725 TSS_725 TSS_724 TSS_724 cstAp1 cstAp1 entHp entHp

Gene      
Name: entH    Texpresso search in the literature
Synonym(s): ECK0590, EG11105, b0597, ybdB
Genome position(nucleotides): 629300 --> 629713
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.76
External database links:  
ASAP:
 ABE-0002057
ECHOBASE:
 EB1097
ECOLIHUB:
 entH
OU-MICROARRAY:
 b0597
STRING:
 511145.b0597
COLOMBOS: entH


Product      
Name: proofreading thioesterase in enterobactin biosynthesis
Synonym(s): EntH, YbdB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 14.97
Isoelectric point: 6.62
Motif(s):
 
Type Positions Sequence Comment
48 -> 48 Q UniProt: 290-fold decrease in activity toward salicylyl-CoA..
50 -> 127 FGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVCQPLHLGRQNQSWEIVVFDEQGRRCC
54 -> 54 H UniProt: 229-fold decrease in activity toward salicylyl-CoA. Loss of activity toward benzoyl-CoA..
63 -> 63 E UniProt: Loss of activity..
64 -> 64 T UniProt: 13-fold decrease in activity toward salicylyl-CoA..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.14 - enterochelin (enterobactin)
  5 - cell processes --> 5.5 - adaptations --> 5.5.7 - Fe aquisition
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016788 - hydrolase activity, acting on ester bonds
GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0016790 - thiolester hydrolase activity
GO:0016289 - CoA hydrolase activity
GO:0042802 - identical protein binding
GO:0061522 - 1,4-dihydroxy-2-naphthoyl-CoA thioesterase activity
biological_process GO:0009239 - enterobactin biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0A8Y8
DIP:
 DIP-11343N
ECOCYC:
 EG11105-MONOMER
ECOLIWIKI:
 b0597
INTERPRO:
 IPR029069
INTERPRO:
 IPR026576
INTERPRO:
 IPR006683
INTERPRO:
 IPR003736
MODBASE:
 P0A8Y8
PDB:
 4K4D
PDB:
 4K4C
PDB:
 1VH9
PFAM:
 PF03061
PRIDE:
 P0A8Y8
REFSEQ:
 NP_415129
SMR:
 P0A8Y8
SWISSMODEL:
 P0A8Y8
UNIPROT:
 P0A8Y8


Operon      
Name: entCEBAH         
Operon arrangement:
Transcription unit        Promoter
entCEBA-ybdB
entH


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: Fur


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_724 629856 forward nd [RS-EPT-CBR] [1]
  promoter TSS_725 629858 forward nd [RS-EPT-CBR] [1]
  promoter TSS_726 629865 forward nd [RS-EPT-CBR] [1]
  promoter TSS_727 629905 forward nd [RS-EPT-CBR] [1]
  promoter ybdDp2 631991 forward nd [ICWHO] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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