RegulonDB RegulonDB 11.1: Gene Form
   

hyfA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hyfB hyfC hyfA bcp FNR FhlA HyfR CRP hyfAp hyfAp

Gene      
Name: hyfA    Texpresso search in the literature
Synonym(s): ECK2477, EG11150, b2481, yffE
Genome position(nucleotides): 2601201 --> 2601818
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.31
Reference(s): [1] Andrews SC., et al., 1997
External database links:  
ASAP:
 ABE-0008179
ECHOBASE:
 EB1139
ECOLIHUB:
 hyfA
OU-MICROARRAY:
 b2481
STRING:
 511145.b2481
COLOMBOS: hyfA


Product      
Name: hydrogenase 4 component A
Synonym(s): HyfA, YffE
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 22.154
Isoelectric point: 6.478
Motif(s):
 
Type Positions Sequence Comment
2 -> 31 NRFVVAEPLWCTGCNTCLAACSDVHKTQGL UniProt: 4Fe-4S ferredoxin-type 1.
41 -> 72 KTSTITAPVVCHHCEEAPCLQVCPVNAISQRD UniProt: 4Fe-4S ferredoxin-type 2.
51 -> 67 CHHCEEAPCLQVCPVNA
73 -> 102 DAIQLNESLCIGCKLCAVVCPFGAISASGS UniProt: 4Fe-4S ferredoxin-type 3.
76 -> 98 QLNESLCIGCKLCAVVCPFGAIS

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0044569 - [Ni-Fe] hydrogenase complex
molecular_function GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
Note(s): Note(s): ...[more].
Reference(s): [1] Andrews SC., et al., 1997
External database links:  
ALPHAFOLD:
 P23481
DIP:
 DIP-9983N
ECOCYC:
 MONOMER0-152
ECOLIWIKI:
 b2481
INTERPRO:
 IPR017900
INTERPRO:
 IPR017896
MODBASE:
 P23481
PFAM:
 PF00037
PFAM:
 PF12798
PRIDE:
 P23481
PRODB:
 PRO_000022963
PROSITE:
 PS51379
PROSITE:
 PS00198
REFSEQ:
 NP_416976
SMR:
 P23481
UNIPROT:
 P23481


Operon      
Name: hyfABCDEFGHIJR-focB         
Operon arrangement:
Transcription unit        Promoter
hyfABCDEFGHIJR-focB


Transcriptional Regulation      
Display Regulation             
Activated by: FhlA, CRP, HyfR
Repressed by: FNR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Andrews SC., Berks BC., McClay J., Ambler A., Quail MA., Golby P., Guest JR., 1997, A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system., Microbiology 143 ( Pt 11):3633-47

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