RegulonDB RegulonDB 10.10: Gene Form
   

murI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

btuB rrsB trmA murI Lrp Lrp H-NS H-NS Lrp Fis H-NS Fis Fis GadX GadX GadX Fis DksA DksA-ppGpp DksA-ppGpp ppGpp Cis-reg; riboswitch TSS_4697 TSS_4697 rrsBp2 rrsBp2 rrsBp1 rrsBp1 TSS_4696 TSS_4696 TSS_4695 TSS_4695 btuBp btuBp trmAp trmAp TSS_4694 TSS_4694 TSS_4693 TSS_4693

Gene      
Name: murI    Texpresso search in the literature
Synonym(s): ECK3959, EG11204, b3967, dga, glr, yijA
Genome position(nucleotides): 4165428 --> 4166285 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.52
External database links:  
ASAP:
 ABE-0012989
CGSC:
 29401
ECHOBASE:
 EB1189
ECOLIHUB:
 murI
OU-MICROARRAY:
 b3967
STRING:
 511145.b3967
COLOMBOS: murI


Product      
Name: glutamate racemase
Synonym(s): Dga, Glr, MurI, YijA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 31.002
Isoelectric point: 5.041
Motif(s):
 
Type Positions Sequence
93 -> 94 NT
113 -> 119 GVVPAIK
25 -> 234 LVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEMLGSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARR
28 -> 29 DS
205 -> 206 TH

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.7 - murein (peptidoglycan)
  6 - cell structure --> 6.2 - murein
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016853 - isomerase activity
GO:0016855 - racemase and epimerase activity, acting on amino acids and derivatives
GO:0008881 - glutamate racemase activity
GO:0047661 - amino-acid racemase activity
GO:0036361 - racemase activity, acting on amino acids and derivatives
biological_process GO:0006807 - nitrogen compound metabolic process
GO:0009252 - peptidoglycan biosynthetic process
GO:0008360 - regulation of cell shape
GO:0071555 - cell wall organization
Note(s): Note(s): ...[more].
Reference(s): [1] Ashiuchi M., et al., 1995
[2] Baliko G., et al., 1988
[3] Hufton SE., et al., 1995
[4] Liu L., et al., 1998
[5] Mathis AD., et al., 2020
[6] Pucci MJ., et al., 1994
[7] Whalen KL., et al., 2013
External database links:  
DIP:
 DIP-10283N
ECOCYC:
 GLUTRACE-MONOMER
ECOLIWIKI:
 b3967
INTERPRO:
 IPR001920
INTERPRO:
 IPR033134
INTERPRO:
 IPR004391
INTERPRO:
 IPR015942
INTERPRO:
 IPR018187
MODBASE:
 P22634
PANTHER:
 PTHR21198:SF2
PDB:
 2JFN
PFAM:
 PF01177
PRIDE:
 P22634
PRODB:
 PRO_000023319
PROSITE:
 PS00924
PROSITE:
 PS00923
REFSEQ:
 NP_418402
SMR:
 P22634
UNIPROT:
 P22634


Operon      
Name: btuB-murI         
Operon arrangement:
Transcription unit        Promoter
btuB-murI


Transcriptional Regulation      
Display Regulation             
Repressed by: GadX


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4693 4162891 reverse nd [RS-EPT-CBR] [8]
  promoter TSS_4694 4162924 reverse nd [RS-EPT-CBR] [8]
  promoter TSS_4695 4163652 forward nd [RS-EPT-CBR] [8]
  promoter TSS_4696 4166020 forward nd [RS-EPT-CBR] [8]
  promoter TSS_4697 4166544 forward nd [RS-EPT-CBR] [8]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Ashiuchi M., Yoshimura T., Kitamura T., Kawata Y., Nagai J., Gorlatov S., Esaki N., Soda K., 1995, In vivo effect of GroESL on the folding of glutamate racemase of Escherichia coli., J Biochem 117(3):495-8

 [2] Baliko G., Raukas A., Boros I., Venetianer P., 1988, An Escherichia coli gene in search of a function., Mol Gen Genet 211(2):326-31

 [3] Hufton SE., Ward RJ., Bunce NA., Armstrong JT., Fletcher AJ., Glass RE., 1995, Structure-function analysis of the vitamin B12 receptor of Escherichia coli by means of informational suppression., Mol Microbiol 15(2):381-93

 [4] Liu L., Yoshimura T., Endo K., Kishimoto K., Fuchikami Y., Manning JM., Esaki N., Soda K., 1998, Compensation for D-glutamate auxotrophy of Escherichia coli WM335 by D-amino acid aminotransferase gene and regulation of murI expression., Biosci Biotechnol Biochem 62(1):193-5

 [5] Mathis AD., Otto RM., Reynolds KA., 2020, A simplified strategy for titrating gene expression reveals new relationships between genotype, environment, and bacterial growth., Nucleic Acids Res

 [6] Pucci MJ., Novotny J., Discotto LF., Dougherty TJ., 1994, The Escherichia coli Dga (MurI) protein shares biological activity and structural domains with the Pediococcus pentosaceus glutamate racemase., J Bacteriol 176(2):528-30

 [7] Whalen KL., Chau AC., Spies MA., 2013, In silico optimization of a fragment-based hit yields biologically active, high-efficiency inhibitors for glutamate racemase., ChemMedChem 8(10):1681-9

 [8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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