RegulonDB

Gene
Name:	pyrL
Synonym(s):	ECK4241, EG11279, b4246, pyrE-, pyrE-LP
Genome position(nucleotides):	4472399 <-- 4472533
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.37
External database links:

ASAP:	ABE-0013899
ECHOBASE:	EB1256
ECOLIHUB:	pyrL
OU-MICROARRAY:	b4246
STRING:	511145.b4246
COLOMBOS:	pyrL

Product

Name:

pyrBI operon leader peptide

Synonym(s):

PyrL

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

5.098

Isoelectric point:

11.959

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 44	MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.2 - pyrimidine biosynthesis
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	biological_process	GO:0006221 - pyrimidine nucleotide biosynthetic process GO:0031555 - transcriptional attenuation GO:0019856 - pyrimidine nucleobase biosynthetic process

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IDA] Inferred from direct assay
[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Levin HL., et al., 1985

External database links:

ALPHAFOLD:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

pyrLBI

Operon arrangement:

Transcription unit	Promoter
pyrLBI	pyrLp2
pyrLBI	pyrLp1

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_5089	4471479	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_5090 (cluster)	4471486	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_5091	4471513	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_5092 (cluster)	4471516	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_5093	4471843	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_5094	4472421	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_5095	4472551	reverse	nd	[RS-EPT-CBR]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Levin HL., Schachman HK., 1985, Regulation of aspartate transcarbamoylase synthesis in Escherichia coli: analysis of deletion mutations in the promoter region of the pyrBI operon., Proc Natl Acad Sci U S A 82(14):4643-7
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Levin HL., Schachman HK., 1985, Regulation of aspartate transcarbamoylase synthesis in Escherichia coli: analysis of deletion mutations in the promoter region of the pyrBI operon., Proc Natl Acad Sci U S A 82(14):4643-7

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.