RegulonDB RegulonDB 11.1: Gene Form
   

leuL gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

leuA leuO leuL RcsB-BglJ LeuO LeuO H-NS SlyA SlyA LeuO LeuO LrhA ppGpp ppGpp Cis-reg; leader anti-anti-terminator anti-terminator terminator leuOp2 leuOp2 leuOp3 leuOp3 leuOp leuOp leuOp1 leuOp1 leuLp2 leuLp2 leuLp1 leuLp1 TSS_146 TSS_146 TSS_145 TSS_145 TSS_144 (cluster) TSS_144 (cluster) TSS_143 TSS_143 TSS_142 TSS_142 TSS_141 (cluster) TSS_141 (cluster) TSS_140 TSS_140 TSS_139 TSS_139 TSS_138 TSS_138 TSS_137 TSS_137 TSS_136 TSS_136 TSS_135 TSS_135 TSS_134 (cluster) TSS_134 (cluster) TSS_133 TSS_133

Gene      
Name: leuL    Texpresso search in the literature
Synonym(s): ECK0077, EG11280, b0075, leuIP, leuLP, leulL, leulLP
Genome position(nucleotides): 83622 <-- 83708
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 47.13
External database links:  
ASAP:
 ABE-0000269
ECHOBASE:
 EB1257
ECOLIHUB:
 leuL
OU-MICROARRAY:
 b0075
STRING:
 511145.b0075
COLOMBOS: leuL


Product      
Name: leu operon leader peptide
Synonym(s): LeuL
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 3.146
Isoelectric point: 12.983
Motif(s):
 
Type Positions Sequence Comment
1 -> 28 MTHIVRFIGLLLLNASSLRGRRVSGIQH

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.19 - leucine
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009082 - branched-chain amino acid biosynthetic process
GO:0009098 - leucine biosynthetic process
GO:0031556 - transcriptional attenuation by ribosome
Note(s): Note(s): ...[more].
Evidence: [AS-HYPO] Author hypothesis
Reference(s): [1] Wessler SR., et al., 1981
External database links:  
ALPHAFOLD:
 P0AD79
ECOCYC:
 EG11280-MONOMER
ECOLIWIKI:
 b0075
INTERPRO:
 IPR012570
PFAM:
 PF08054
PRIDE:
 P0AD79
REFSEQ:
 NP_414617
UNIPROT:
 P0AD79


Operon      
Name: leuLABCD         
Operon arrangement:
Transcription unit        Promoter
leuL
leuLABCD
leuLABCD


Transcriptional Regulation      
Display Regulation             
Activated by: LeuO


RNA cis-regulatory element    
   
Cis-reg; leader Leucine operon leader
   
   


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_133 82058 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_134 (cluster) 82082 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_135 82123 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_136 82207 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_137 82255 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_138 82361 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_139 82593 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_140 82671 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_141 (cluster) 82704 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_142 83028 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_143 83030 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_144 (cluster) 83034 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_145 83349 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_146 83728 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Wessler SR., Calvo JM., 1981, Control of leu operon expression in Escherichia coli by a transcription attenuation mechanism., J Mol Biol 149(4):579-97

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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