RegulonDB RegulonDB 10.8: Gene Form
   

acnA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

acnA ribA ymiC ArcA FNR CRP SoxS Rob MarA acnAp3 acnAp3 acnAp2 acnAp2

Gene      
Name: acnA    Texpresso search in the literature
Synonym(s): ECK1271, EG11325, acn, b1276
Genome position(nucleotides): 1335831 --> 1338506 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.91
External database links:  
ASAP:
 ABE-0004283
CGSC:
 28218
ECHOBASE:
 EB1301
OU-MICROARRAY:
 b1276
PortEco:
 acnA
STRING:
 511145.b1276
COLOMBOS: acnA


Product      
Name: aconitate hydratase 1
Synonym(s): Acn, AcnA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 97.677
Isoelectric point: 5.677
Motif(s):
 
Type Positions Sequence
2 -> 891 SSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKVLLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIKKSDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGINVTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK
69 -> 562 WLKNAHADREIAYRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIKKSDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAG
522 -> 522 S
691 -> 818 RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQ

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
  3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional
  5 - cell processes --> 5.5 - adaptations --> 5.5.6 - other (mechanical, nutritional, oxidative stress)
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0047780 - citrate dehydratase activity
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0003723 - RNA binding
GO:0003729 - mRNA binding
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0003994 - aconitate hydratase activity
GO:0005506 - iron ion binding
GO:0003730 - mRNA 3'-UTR binding
biological_process GO:0006097 - glyoxylate cycle
GO:0006099 - tricarboxylic acid cycle
GO:0006979 - response to oxidative stress
GO:0009061 - anaerobic respiration
GO:0006101 - citrate metabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Prodromou C., et al., 1992
[2] Prodromou C., et al., 1991
External database links:  
DIP:
 DIP-9043N
ECOCYC:
 ACONITASE-MONOMER
ECOLIWIKI:
 b1276
INTERPRO:
 IPR006249
INTERPRO:
 IPR036008
INTERPRO:
 IPR018136
INTERPRO:
 IPR015931
INTERPRO:
 IPR015928
INTERPRO:
 IPR001030
INTERPRO:
 IPR000573
MINT:
 MINT-1249275
MODBASE:
 P25516
PANTHER:
 PTHR11670
PFAM:
 PF00330
PFAM:
 PF00694
PRIDE:
 P25516
PRINTS:
 PR00415
PRODB:
 PRO_000022042
PROSITE:
 PS01244
PROSITE:
 PS00450
REFSEQ:
 NP_415792
SMR:
 P25516
UNIPROT:
 P25516


Operon      
Name: ymiC-acnA         
Operon arrangement:
Transcription unit        Promoter
acnA
acnA
acnA


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, MarA, Cra, SoxS, Rob
Repressed by: FNR, ArcA
Growth Conditions:

[1] 

C: Escherichia coli| soxR knockout mutant; soxS knockout mutant; plasmid pSE380 mutant| LB medium| paraquat 50 µM| 37.0 C
E: Escherichia coli| soxR knockout mutant; soxS knockout mutant; plasmid pSXS (IPTG-induced soxS) mutant| LB medium| paraquat 50 µM| 37.0 C

[2] 

C: Escherichia coli| soxR knockout mutant; soxS knockout mutant; plasmid pSXR (IPTG-induced soxR) mutant| LB medium| 37.0 C
E: Escherichia coli| soxR knockout mutant; soxS knockout mutant; plasmid pSXR (IPTG-induced soxR) mutant| LB medium| paraquat 50 µM| 37.0 C

[3] 

C: Escherichia coli| soxR knockout mutant; soxS knockout mutant; plasmid pSE380 mutant| LB medium| 2,2'-bipyridine 150 ?m| 37.0 C
E: Escherichia coli| soxR knockout mutant; soxS knockout mutant; plasmid pSXR (IPTG-induced soxR) mutant| LB medium| 2,2'-bipyridine 150 ?m| 37.0 C

[4] 

C: Escherichia coli| soxR knockout mutant; soxS knockout mutant; plasmid pSE380 mutant| LB medium| 2,2'-bipyridine 150 ?m| 37.0 C
E: Escherichia coli| soxR knockout mutant; soxS knockout mutant; plasmid pSXS (IPTG-induced soxS) mutant| LB medium| 2,2'-bipyridine 150 ?m| 37.0 C


Regulation by small RNA    
  Display Regulation
small RNA ryhB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Prodromou C., Artymiuk PJ., Guest JR., 1992, The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases., Eur J Biochem 204(2):599-609

 [2] Prodromou C., Haynes MJ., Guest JR., 1991, The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn)., J Gen Microbiol 137(11):2505-15

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