RegulonDB

Gene
Name:	acnA
Synonym(s):	ECK1271, EG11325, acn, b1276
Genome position(nucleotides):	1335831 --> 1338506
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.91
External database links:

ASAP:	ABE-0004283
CGSC:	28218
ECHOBASE:	EB1301
ECOLIHUB:	acnA
OU-MICROARRAY:	b1276
STRING:	511145.b1276
COLOMBOS:	acnA

Product

Name:

aconitate hydratase 1

Synonym(s):

Acn, AcnA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

97.677

Isoelectric point:

5.677

Motif(s):

Type	Positions	Sequence	Comment
	2 -> 891	SSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKVLLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIKKSDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGINVTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK	UniProt: Aconitate hydratase A.
	69 -> 562	WLKNAHADREIAYRPARVLMQDFTGVPAVVDLAAMREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFLKWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETAIKKSDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAG
	522 -> 522	S	UniProt: In Ref. 1; CAA42834 and 4; BAA14828..
	691 -> 818	RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQ

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional
		5 - cell processes --> 5.5 - adaptations --> 5.5.6 - other (mechanical, nutritional, oxidative stress)
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0047780 - citrate dehydratase activity GO:0016829 - lyase activity GO:0046872 - metal ion binding GO:0003723 - RNA binding GO:0003729 - mRNA binding GO:0051536 - iron-sulfur cluster binding GO:0051539 - 4 iron, 4 sulfur cluster binding GO:0003994 - aconitate hydratase activity GO:0005506 - iron ion binding GO:0003730 - mRNA 3'-UTR binding
	biological_process	GO:0006097 - glyoxylate cycle GO:0006099 - tricarboxylic acid cycle GO:0006979 - response to oxidative stress GO:0009061 - anaerobic respiration GO:0006101 - citrate metabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Prodromou C., et al., 1992
[2] Prodromou C., et al., 1991

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, MarA, Cra, Rob, SoxS
Repressed by:	FNR, ArcA

Reference(s)
[1] Prodromou C., Artymiuk PJ., Guest JR., 1992, The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases., Eur J Biochem 204(2):599-609
[2] Prodromou C., Haynes MJ., Guest JR., 1991, The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn)., J Gen Microbiol 137(11):2505-15