RegulonDB RegulonDB 11.1: Gene Form
   

lysP gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cirA lysP yeiE ArgP ArgP Fur Fur Fur Fur CRP RyhB TSS_2459 TSS_2459 lysPp lysPp cirAp2 cirAp2 cirAp1 cirAp1

Gene      
Name: lysP    Texpresso search in the literature
Synonym(s): ECK2149, EG11337, b2156, cadR
Genome position(nucleotides): 2247063 <-- 2248532
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.47
External database links:  
ASAP:
 ABE-0007131
CGSC:
 942
ECHOBASE:
 EB1313
ECOLIHUB:
 lysP
OU-MICROARRAY:
 b2156
STRING:
 511145.b2156
COLOMBOS: lysP


Product      
Name: lysine:H+ symporter
Synonym(s): CadR, LysP, lysine specific permease, lysine transporter, lysine:proton symporter
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space,inner membrane
Molecular weight: 53.576
Isoelectric point: 9.033
Motif(s):
 
Type Positions Sequence Comment
2 -> 489 VSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATISQAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNYWYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFSLIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSFQGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDVKDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPRIFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAISHYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGVAATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK UniProt: Lysine-specific permease.
21 -> 476 HLTMIAIGGSIGTGLFVASGATISQAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNYWYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFSLIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSFQGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDVKDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPRIFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAISHYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGVAATYIGIPLFLIIWFGYKLIKGTHFV
22 -> 42 LTMIAIGGSIGTGLFVASGAT UniProt: Helical.
56 -> 76 MLIGLMVYFLMTSLGELAAYM UniProt: Helical.
102 -> 102 Y Y → L: significant decrease in cadBA expression under inducing conditions; significant defect in lysine transport (11-fold increase in Km and 14-fold reduction in Vmax)

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.7 - lysine
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0008134 - transcription factor binding
GO:0015171 - amino acid transmembrane transporter activity
GO:0015189 - L-lysine transmembrane transporter activity
GO:0042802 - identical protein binding
biological_process GO:0097639 - L-lysine import across plasma membrane
GO:0051090 - regulation of DNA-binding transcription factor activity
GO:0006865 - amino acid transport
GO:0055085 - transmembrane transport
GO:0043200 - response to amino acid
GO:0003333 - amino acid transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Brameyer S., et al., 2019
[2] Fritz G., et al., 2009
[3] Neely MN., et al., 1996
[4] Rosen BP. 1971
[5] Ruiz J., et al., 2011
External database links:  
ALPHAFOLD:
 P25737
ECOCYC:
 LYSP-MONOMER
ECOLIWIKI:
 b2156
INTERPRO:
 IPR004840
INTERPRO:
 IPR004841
PFAM:
 PF00324
PRIDE:
 P25737
PRODB:
 PRO_000023139
PROSITE:
 PS00218
REFSEQ:
 NP_416661
UNIPROT:
 P25737


Operon      
Name: lysP         
Operon arrangement:
Transcription unit        Promoter
lysP


Transcriptional Regulation      
Display Regulation             
Activated by: ArgP, Lrp


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2459 2249471 reverse nd [RS-EPT-CBR] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Brameyer S., Rosch TC., El Andari J., Hoyer E., Schwarz J., Graumann PL., Jung K., 2019, DNA-binding directs the localization of a membrane-integrated receptor of the ToxR family., Commun Biol 2:4

 [2] Fritz G., Koller C., Burdack K., Tetsch L., Haneburger I., Jung K., Gerland U., 2009, Induction kinetics of a conditional pH stress response system in Escherichia coli., J Mol Biol 393(2):272-86

 [3] Neely MN., Olson ER., 1996, Kinetics of expression of the Escherichia coli cad operon as a function of pH and lysine., J Bacteriol 178(18):5522-8

 [4] Rosen BP., 1971, Basic amino acid transport in Escherichia coli., J Biol Chem 246(11):3653-62

 [5] Ruiz J., Haneburger I., Jung K., 2011, Identification of ArgP and Lrp as transcriptional regulators of lysP, the gene encoding the specific lysine permease of Escherichia coli., J Bacteriol 193(10):2536-48

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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