RegulonDB RegulonDB 11.1: Gene Form
   

mdtG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdtG lpxL msyB b1052 anti-anti-terminator anti-terminator terminator TSS_1332 TSS_1332 msyBp msyBp TSS_1331 TSS_1331

Gene      
Name: mdtG    Texpresso search in the literature
Synonym(s): ECK1038, EG11343, b1053, yceE
Genome position(nucleotides): 1114264 <-- 1115490
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.49
External database links:  
ASAP:
 ABE-0003572
ECHOBASE:
 EB1319
ECOLIHUB:
 mdtG
OU-MICROARRAY:
 b1053
STRING:
 511145.b1053
COLOMBOS: mdtG


Product      
Name: efflux pump MdtG
Synonym(s): MdtG, MdtG drug MFS transporter, MdtG multidrug efflux pump, YceE
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 43.867
Isoelectric point: 10.847
Motif(s):
 
Type Positions Sequence Comment
16 -> 36 LIVAWLGCFLTGAAFSLVMPF UniProt: Helical.
20 -> 263 WLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPMAGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMI
58 -> 78 IVFSITFLFSAIASPFWGGLA UniProt: Helical.
92 -> 112 LGMGIVMVLMGLAQNIWQFLI UniProt: Helical.
115 -> 135 ALLGLLGGFVPNANALIATQV UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0022857 - transmembrane transporter activity
GO:0015299 - solute:proton antiporter activity
biological_process GO:1990961 - xenobiotic detoxification by transmembrane export across the plasma membrane
GO:0046677 - response to antibiotic
GO:0055085 - transmembrane transport
GO:1901562 - response to paraquat
Note(s): Note(s): ...[more].
Reference(s): [1] Jindal S., et al., 2019
External database links:  
ALPHAFOLD:
 P25744
ECOCYC:
 YCEE-MONOMER
ECOLIWIKI:
 b1053
INTERPRO:
 IPR023692
INTERPRO:
 IPR036259
INTERPRO:
 IPR020846
INTERPRO:
 IPR011701
INTERPRO:
 IPR001958
MODBASE:
 P25744
PFAM:
 PF07690
PRIDE:
 P25744
PRINTS:
 PR01035
PRODB:
 PRO_000023187
PROSITE:
 PS50850
REFSEQ:
 NP_415571
UNIPROT:
 P25744


Operon      
Name: mdtG         
Operon arrangement:
Transcription unit        Promoter
mdtG


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1331 1114212 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1332 1115544 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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