RegulonDB RegulonDB 11.1: Gene Form
   

bcr gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

bcr rsuA yejG unknown TSS_2478 TSS_2478 TSS_2477 TSS_2477 yejGp8 yejGp8 yejGp2 yejGp2 yejGp5 yejGp5 yejGp1 yejGp1

Gene      
Name: bcr    Texpresso search in the literature
Synonym(s): ECK2176, EG11419, b2182, bicA, bicR, sur, suxA
Genome position(nucleotides): 2278570 <-- 2279760
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.32
External database links:  
ASAP:
 ABE-0007221
CGSC:
 32582
ECHOBASE:
 EB1390
ECOLIHUB:
 bcr
OU-MICROARRAY:
 b2182
STRING:
 511145.b2182
COLOMBOS: bcr


Product      
Name: multidrug efflux pump Bcr
Synonym(s): Bcr, BicA, BicR, Sur, SuxA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 43.353
Isoelectric point: 10.101
Motif(s):
 
Type Positions Sequence Comment
9 -> 26 FAIVFILGLLAMLMPLSI UniProt: Helical.
16 -> 358 GLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVG
49 -> 65 MTLSTYILGFALGQLIY UniProt: Helical.
77 -> 98 VVLGGTLVFAAAAVACALANTI UniProt: Helical.
99 -> 99 D UniProt: In Ref. 2; CAA45230..

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0022857 - transmembrane transporter activity
GO:0015299 - solute:proton antiporter activity
GO:0015385 - sodium:proton antiporter activity
GO:0042910 - xenobiotic transmembrane transporter activity
GO:0033229 - cysteine transmembrane transporter activity
GO:0071916 - dipeptide transmembrane transporter activity
biological_process GO:1990961 - xenobiotic detoxification by transmembrane export across the plasma membrane
GO:0046677 - response to antibiotic
GO:0015031 - protein transport
GO:0015833 - peptide transport
GO:0055085 - transmembrane transport
GO:0042908 - xenobiotic transport
GO:0033228 - cysteine export across plasma membrane
GO:0035725 - sodium ion transmembrane transport
GO:0035442 - dipeptide transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Jindal S., et al., 2019
External database links:  
ALPHAFOLD:
 P28246
ECOCYC:
 BCR-MONOMER
ECOLIWIKI:
 b2182
INTERPRO:
 IPR004734
INTERPRO:
 IPR011701
INTERPRO:
 IPR020846
INTERPRO:
 IPR004812
INTERPRO:
 IPR036259
MODBASE:
 P28246
PFAM:
 PF07690
PRIDE:
 P28246
PRODB:
 PRO_000022201
PROSITE:
 PS50850
REFSEQ:
 NP_416687
UNIPROT:
 P28246


Operon      
Name: bcr         
Operon arrangement:
Transcription unit        Promoter
bcr


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yejGp5 2278353 reverse nd [COMP-AINF] [2]
  promoter yejGp8 2278446 reverse nd [COMP-AINF] [2]
  promoter TSS_2477 2278466 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2478 2279068 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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