RegulonDB RegulonDB 10.9: Gene Form
   

rffG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

wecC wecB rffG rffH rffC TSS_4444 TSS_4444

Gene      
Name: rffG    Texpresso search in the literature
Synonym(s): ECK3780, EG11453, b3788, rff
Genome position(nucleotides): 3972522 --> 3973589 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.75
External database links:  
ASAP:
ABE-0012376
CGSC:
33925
ECHOBASE:
EB1422
ECOLIHUB:
rffG
OU-MICROARRAY:
b3788
STRING:
511145.b3788
COLOMBOS: rffG


Product      
Name: dTDP-glucose 4,6-dehydratase 2
Synonym(s): Rff, RffG
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 39.754
Isoelectric point: 5.893
Motif(s):
 
Type Positions Sequence
33 -> 36 DKLT
314 -> 315 WL
81 -> 85 LAAES
215 -> 217 PVY
160 -> 164 YSASK

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.7 - nicotinamide adenine dinucleotide
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.1 - O antigen
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.4 - enterobacterial common antigen (surface glycolipid)
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016829 - lyase activity
GO:0008460 - dTDP-glucose 4,6-dehydratase activity
biological_process GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009225 - nucleotide-sugar metabolic process
GO:0009246 - enterobacterial common antigen biosynthetic process
GO:0045226 - extracellular polysaccharide biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ECOCYC:
DTDPGLUCDEHYDRAT2-MONOMER
ECOLIWIKI:
b3788
INTERPRO:
IPR016040
INTERPRO:
IPR036291
INTERPRO:
IPR005888
MODBASE:
P27830
PDB:
1BXK
PFAM:
PF16363
PRIDE:
P27830
REFSEQ:
YP_026255
SMR:
P27830
UNIPROT:
P27830


Operon      
Name: rfe-wzzE-wecBC-rffGHC-wecE-wzxE-wecF-wzyE-rffM         
Operon arrangement:
Transcription unit        Promoter
rfe-wzzE-wecBC-rffGHC-wecE-wzxE-wecF-wzyE-rffM


Transcriptional Regulation      
Display Regulation             
Repressed by: NsrR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4444 3971398 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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