RegulonDB

Gene
Name:	mliC
Synonym(s):	ECK1635, EG11488, b1639, ydhA
Genome position(nucleotides):	1718066 <-- 1718395 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	45.76
External database links:

ASAP:	ABE-0005485
ECHOBASE:	EB1451
ECOLIHUB:	mliC
OU-MICROARRAY:	b1639
STRING:	511145.b1639
COLOMBOS:	mliC

Product

Name:

inhibitor of c-type lysozyme, putative lipoprotein

Synonym(s):

MliC, YdhA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane

Molecular weight:

12.568

Isoelectric point:

8.463

Motif(s):

Type	Positions	Sequence
	1 -> 17	MTMKKLLIIILPVLLSG
	35 -> 98	YQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIV
	18 -> 109	CSAFNQLVERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNCQLQNPQR

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
	cellular_component	GO:0009279 - cell outer membrane GO:0016020 - membrane GO:0031241 - periplasmic side of cell outer membrane
	molecular_function	GO:0005515 - protein binding GO:0060241 - lysozyme inhibitor activity
	biological_process	GO:0043086 - negative regulation of catalytic activity

Note(s):

Note(s): ...[more].

Evidence:

[APPP] Assay of partially-purified protein

Reference(s):

[1] Callewaert L., et al., 2008
[2] Rao S., et al., 2020

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PDB:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

mliC

Operon arrangement:

Transcription unit	Promoter
mliC

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_1915	1717731		reverse	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Callewaert L., Aertsen A., Deckers D., Vanoirbeek KG., Vanderkelen L., Van Herreweghe JM., Masschalck B., Nakimbugwe D., Robben J., Michiels CW., 2008, A new family of lysozyme inhibitors contributing to lysozyme tolerance in gram-negative bacteria., PLoS Pathog 4(3):e1000019
[2] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.