RegulonDB RegulonDB 11.2: Gene Form
   

nadC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nadC ampD ppdD ampE CRP CRP terminator ampD ampD ampDp5 ampDp5 nadCp nadCp TSS_232 TSS_232 ppdDp ppdDp

Gene      
Name: nadC    Texpresso search in the literature
Synonym(s): ECK0108, EG11546, b0109
Genome position(nucleotides): 117752 <-- 118645
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.13
External database links:  
ASAP:
 ABE-0000374
CGSC:
 465
ECHOBASE:
 EB1508
ECOLIHUB:
 nadC
OU-MICROARRAY:
 b0109
STRING:
 511145.b0109
COLOMBOS: nadC


Product      
Name: quinolinate phosphoribosyltransferase (decarboxylating)
Synonym(s): NadC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.762
Isoelectric point: 4.838
Motif(s):
 
Type Positions Sequence Comment
2 -> 297 PPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANNDITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQSLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLRSALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVENLEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVDFISVGALTKHVQALDLSMRFR UniProt: Nicotinate-nucleotide pyrophosphorylase [carboxylating].
43 -> 129 NDITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQSLFELEGPSRVLLTGERTALNFVQTLS
119 -> 119 R UniProt: In Ref. 2; AAB00467..
131 -> 295 VASKVRHYVELLEGTNTQLLDTRKTLPGLRSALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVENLEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVDFISVGALTKHVQALDLSMR
152 -> 154 TRK UniProt: Substrate binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.7 - nicotinamide adenine dinucleotide
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016757 - glycosyltransferase activity
GO:0016740 - transferase activity
GO:0016763 - pentosyltransferase activity
GO:0004514 - nicotinate-nucleotide diphosphorylase (carboxylating) activity
biological_process GO:0009435 - NAD biosynthetic process
GO:0019363 - pyridine nucleotide biosynthetic process
GO:0034628 - 'de novo' NAD biosynthetic process from aspartate
GO:0034213 - quinolinate catabolic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P30011
DIP:
 DIP-10293N
ECOCYC:
 QUINOPRIBOTRANS-MONOMER
ECOLIWIKI:
 b0109
INTERPRO:
 IPR037128
INTERPRO:
 IPR036068
INTERPRO:
 IPR027277
INTERPRO:
 IPR022412
INTERPRO:
 IPR013785
INTERPRO:
 IPR004393
INTERPRO:
 IPR002638
MODBASE:
 P30011
PANTHER:
 PTHR32179
PFAM:
 PF01729
PFAM:
 PF02749
PRIDE:
 P30011
REFSEQ:
 NP_414651
SMR:
 P30011
SWISSMODEL:
 P30011
UNIPROT:
 P30011


Operon      
Name: nadC         
Operon arrangement:
Transcription unit        Promoter
nadC


Transcriptional Regulation      
Display Regulation             
Activated by: CRP


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_232 118078 reverse nd [RS-EPT-CBR] [1]
  promoter ampDp5 118681 forward nd [COMP-AINF] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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