RegulonDB RegulonDB 11.1: Gene Form
   

thiP gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

thiP thiB yabI thiQ sroA Cis-reg; riboswitch terminator sroAp sroAp thiQp2 thiQp2

Gene      
Name: thiP    Texpresso search in the literature
Synonym(s): ECK0068, EG11573, b0067, sfuB, yabK
Genome position(nucleotides): 72911 <-- 74521
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 58.41
External database links:  
ASAP:
 ABE-0000246
ECHOBASE:
 EB1533
ECOLIHUB:
 thiP
OU-MICROARRAY:
 b0067
STRING:
 511145.b0067
COLOMBOS: thiP


Product      
Name: thiamine ABC transporter membrane subunit
Synonym(s): SfuB, ThiP, YabK
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 59.533
Isoelectric point: 9.399
Motif(s):
 
Type Positions Sequence Comment
12 -> 32 WLIPGVSATTLVVAVALAAFL UniProt: Helical.
56 -> 261 VRFSFWQAFLSALLSVIPAIFLARALYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGWLATLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQALENIPGEQRQLAAQLGMRSWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLS UniProt: ABC transmembrane type-1 1.
58 -> 78 FSFWQAFLSALLSVIPAIFLA UniProt: Helical.
74 -> 266 AIFLARALYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGWLATLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQALENIPGEQRQLAAQLGMRSWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSK
95 -> 115 LCAMTLILPVLVAVFGILSVY UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.8 - thiamin
  6 - cell structure --> 6.1 - membrane
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.m - ABC superfamily, membrane component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0043190 - ATP-binding cassette (ABC) transporter complex
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
molecular_function GO:0022857 - transmembrane transporter activity
GO:0015234 - thiamine transmembrane transporter activity
biological_process GO:0015888 - thiamine transport
GO:0055085 - transmembrane transport
GO:0071934 - thiamine transmembrane transport
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P31549
DIP:
 DIP-10989N
ECOCYC:
 SFUB-MONOMER
ECOLIWIKI:
 b0067
INTERPRO:
 IPR005947
INTERPRO:
 IPR035906
INTERPRO:
 IPR000515
PFAM:
 PF00528
PRIDE:
 P31549
PRODB:
 PRO_000024063
PROSITE:
 PS50928
REFSEQ:
 NP_414609
SMR:
 P31549
UNIPROT:
 P31549


Operon      
Name: sgrR-sroA-thiBPQ         
Operon arrangement:
Transcription unit        Promoter
sroA
sgrR-sroA-thiBPQ


Transcriptional Regulation      
Display Regulation             
Repressed by: SgrR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter thiQp2 73085 reverse nd [COMP-AINF] [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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