RegulonDB RegulonDB 11.1: Gene Form
   

leuC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

leuC leuB leuD TSS_132 TSS_132 TSS_131 (cluster) TSS_131 (cluster) TSS_130 TSS_130 TSS_129 TSS_129 TSS_128 TSS_128 TSS_127 TSS_127 TSS_126 TSS_126 TSS_125 TSS_125 TSS_124 TSS_124 leuDp4 leuDp4 TSS_123 TSS_123 TSS_122 TSS_122

Gene      
Name: leuC    Texpresso search in the literature
Synonym(s): ECK0074, EG11576, b0072
Genome position(nucleotides): 79464 <-- 80864
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.75
External database links:  
ASAP:
 ABE-0000261
CGSC:
 570
ECHOBASE:
 EB1536
ECOLIHUB:
 leuC
OU-MICROARRAY:
 b0072
STRING:
 511145.b0072
COLOMBOS: leuC


Product      
Name: 3-isopropylmalate dehydratase subunit LeuC
Synonym(s): 3-isopropylmalate dehydratase, large subunit, LeuC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 49.882
Isoelectric point: 6.275
Motif(s):
 
Type Positions Sequence Comment
2 -> 466 AKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNIK UniProt: 3-isopropylmalate dehydratase large subunit.
7 -> 457 EKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTG
74 -> 74 A UniProt: In Ref. 5; BAA21004/BAA21005..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.19 - leucine
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0009316 - 3-isopropylmalate dehydratase complex
molecular_function GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0003861 - 3-isopropylmalate dehydratase activity
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009082 - branched-chain amino acid biosynthetic process
GO:0009098 - leucine biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Davis MG., et al., 1977
[2] Parimi NS., et al., 2017
[3] Wu X., et al., 2017
[4] Yang CR., et al., 2005
External database links:  
ALPHAFOLD:
 P0A6A6
DIP:
 DIP-35834N
ECOCYC:
 LEUC-MONOMER
ECOLIWIKI:
 b0072
INTERPRO:
 IPR033941
INTERPRO:
 IPR036008
INTERPRO:
 IPR018136
INTERPRO:
 IPR015931
INTERPRO:
 IPR004430
INTERPRO:
 IPR001030
PFAM:
 PF00330
PRIDE:
 P0A6A6
PRINTS:
 PR00415
PRODB:
 PRO_000023083
PROSITE:
 PS00450
PROSITE:
 PS01244
REFSEQ:
 NP_414614
UNIPROT:
 P0A6A6


Operon      
Name: leuLABCD         
Operon arrangement:
Transcription unit        Promoter
leuL
leuLABCD
leuLABCD


Transcriptional Regulation      
Display Regulation             
Activated by: LeuO


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_122 79281 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_123 79386 reverse nd [RS-EPT-CBR] [5]
  promoter leuDp4 79594 reverse nd [COMP-AINF] [6]
  promoter TSS_124 79955 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_125 80236 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_126 80442 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_127 80589 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_128 80670 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_129 81214 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_130 81440 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_131 (cluster) 81452 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_132 81559 reverse nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Davis MG., Calvo JM., 1977, Isolation and characterization of lambda pleu bacteriophages., J Bacteriol 129(2):1078-90

 [2] Parimi NS., Durie IA., Wu X., Niyas AMM., Eiteman MA., 2017, Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli., Microb Cell Fact 16(1):114

 [3] Wu X., Eiteman MA., 2017, Synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli., J Ind Microbiol Biotechnol 44(10):1483-1490

 [4] Yang CR., Shapiro BE., Hung SP., Mjolsness ED., Hatfield GW., 2005, A mathematical model for the branched chain amino acid biosynthetic pathways of Escherichia coli K12., J Biol Chem 280(12):11224-32

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RegulonDB