RegulonDB RegulonDB 11.1: Gene Form
   

leuB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

leuA leuC leuB leuL Cis-reg; leader anti-anti-terminator anti-terminator terminator TSS_145 TSS_145 TSS_144 (cluster) TSS_144 (cluster) TSS_143 TSS_143 TSS_142 TSS_142 TSS_141 (cluster) TSS_141 (cluster) TSS_140 TSS_140 TSS_139 TSS_139 TSS_138 TSS_138 TSS_137 TSS_137 TSS_136 TSS_136 TSS_135 TSS_135 TSS_134 (cluster) TSS_134 (cluster) TSS_133 TSS_133 TSS_132 TSS_132 TSS_131 (cluster) TSS_131 (cluster) TSS_130 TSS_130 TSS_129 TSS_129 TSS_128 TSS_128 TSS_127 TSS_127 TSS_126 TSS_126 TSS_125 TSS_125 TSS_124 TSS_124 leuDp4 leuDp4

Gene      
Name: leuB    Texpresso search in the literature
Synonym(s): ECK0075, EG11577, b0073
Genome position(nucleotides): 80867 <-- 81958
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.49
External database links:  
ASAP:
 ABE-0000265
CGSC:
 571
ECHOBASE:
 EB1537
ECOLIHUB:
 leuB
OU-MICROARRAY:
 b0073
STRING:
 511145.b0073
COLOMBOS: leuB


Product      
Name: 3-isopropylmalate dehydrogenase
Synonym(s): LeuB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 39.517
Isoelectric point: 4.927
Motif(s):
 
Type Positions Sequence Comment
2 -> 2 S UniProt: In Ref. 6; AA sequence..
6 -> 355 HIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYDVGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHFKLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPDVELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGIRTGDLARGAAAVSTDEMGDII
78 -> 91 GPKWEHLPPDQQPE UniProt: NAD.
90 -> 100 PERGALLPLRK substrate recognition region
285 -> 297 GSAPDIAGKNIAN UniProt: NAD.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.19 - leucine
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0003862 - 3-isopropylmalate dehydrogenase activity
GO:0016616 - oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0000287 - magnesium ion binding
GO:0030145 - manganese ion binding
GO:0051287 - NAD binding
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009082 - branched-chain amino acid biosynthetic process
GO:0009098 - leucine biosynthetic process
GO:0034198 - cellular response to amino acid starvation
External database links:  
ALPHAFOLD:
 P30125
ECOCYC:
 3-ISOPROPYLMALDEHYDROG-MONOMER
ECOLIWIKI:
 b0073
INTERPRO:
 IPR019818
INTERPRO:
 IPR024084
INTERPRO:
 IPR004429
MODBASE:
 P30125
PANTHER:
 PTHR42979
PDB:
 1CM7
PFAM:
 PF00180
PRIDE:
 P30125
PRODB:
 PRO_000023082
PROSITE:
 PS00470
REFSEQ:
 NP_414615
SMART:
 SM01329
SMR:
 P30125
UNIPROT:
 P30125


Operon      
Name: leuLABCD         
Operon arrangement:
Transcription unit        Promoter
leuL
leuLABCD
leuLABCD


Transcriptional Regulation      
Display Regulation             
Activated by: LeuO


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter leuDp4 79594 reverse nd [COMP-AINF] [1]
  promoter TSS_124 79955 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_125 80236 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_126 80442 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_127 80589 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_128 80670 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_129 81214 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_130 81440 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_131 (cluster) 81452 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_132 81559 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_133 82058 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_134 (cluster) 82082 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_135 82123 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_136 82207 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_137 82255 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_138 82361 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_139 82593 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_140 82671 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_141 (cluster) 82704 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_142 83028 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_143 83030 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_144 (cluster) 83034 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_145 83349 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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