RegulonDB

Gene
Name:	eamA
Synonym(s):	ECK1526, EG11639, b1533, ydeD
Genome position(nucleotides):	1620238 <-- 1621137 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.78
Reference(s):	[1] Dassler T., et al., 2000
External database links:

ASAP:	ABE-0005116
ECHOBASE:	EB1594
ECOLIHUB:	eamA
OU-MICROARRAY:	b1533
STRING:	511145.b1533
COLOMBOS:	eamA

Shine dalgarno
Sequence:	tgaaattcagAGGCGGtagAAT

Product

Name:

cysteine/O-acetylserine exporter EamA

Synonym(s):

EamA, YdeD, orf266, orf299

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

32.324

Isoelectric point:

10.452

Motif(s):

Type	Positions	Sequence
	32 -> 52	PLMLAGLRFMLVAFPAIFFVA
	60 -> 80	LLLGYGLTISFAQFAFLFCAI
	117 -> 137	LAGIALAIFGVLVLIEDSLNG
	244 -> 264	VAPLSLLVPVVGLASAALLLD
	153 -> 287	FSWACGNIFNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLMYLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAVLIMTGLYINVF

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0015562 - efflux transmembrane transporter activity
	biological_process	GO:0006865 - amino acid transport GO:0070301 - cellular response to hydrogen peroxide GO:0033228 - cysteine export across plasma membrane

Note(s):

Note(s): ...[more].

Reference(s):

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

eamA

Operon arrangement:

Transcription unit	Promoter
eamA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1841	1621167	reverse	nd	[RS-EPT-CBR]	[4]
promoter	ydeEp4	1621264	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.62. P-value: 5.490e-5 Read more >	[ICWHO]	[5]
promoter	TSS_1842	1621290	forward	nd	[RS-EPT-CBR]	[4]
promoter	ydeEp1	1621304	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 2.89. P-value: 9.234e-5 Read more >	[ICWHO]	[5]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Dassler T., Maier T., Winterhalter C., Bock A., 2000, Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway., Mol Microbiol 36(5):1101-12
[2] Franke I., Resch A., Dassler T., Maier T., Bock A., 2003, YfiK from Escherichia coli promotes export of O-acetylserine and cysteine., J Bacteriol 185(4):1161-6
[3] Maier TH., 2003, Semisynthetic production of unnatural L-alpha-amino acids by metabolic engineering of the cysteine-biosynthetic pathway., Nat Biotechnol 21(4):422-7
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78