RegulonDB RegulonDB 11.1: Gene Form
   

eamA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ydeE eamA marB ydeEp1 ydeEp1 TSS_1842 TSS_1842 ydeEp4 ydeEp4 TSS_1841 TSS_1841

Gene      
Name: eamA    Texpresso search in the literature
Synonym(s): ECK1526, EG11639, b1533, ydeD
Genome position(nucleotides): 1620238 <-- 1621137
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.78
Reference(s): [1] Dassler T., et al., 2000
External database links:  
ASAP:
 ABE-0005116
ECHOBASE:
 EB1594
ECOLIHUB:
 eamA
OU-MICROARRAY:
 b1533
STRING:
 511145.b1533
COLOMBOS: eamA


Shine dalgarno      
Sequence: tgaaattcagAGGCGGtagAAT


Product      
Name: cysteine/O-acetylserine exporter EamA
Synonym(s): EamA, YdeD, orf266, orf299
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 32.324
Isoelectric point: 10.452
Motif(s):
 
Type Positions Sequence Comment
7 -> 27 VLALLVVVVWGLNFVVIKVGL UniProt: Helical.
14 -> 132 VVWGLNFVVIKVGLHNMPPLMLAGLRFMLVAFPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIMLGAFTFGERLHGKQLAGIALAIFGVLVLIE UniProt: EamA 1.
32 -> 52 PLMLAGLRFMLVAFPAIFFVA UniProt: Helical.
60 -> 80 LLLGYGLTISFAQFAFLFCAI UniProt: Helical.
88 -> 108 LASLVLQAQAFFTIMLGAFTF UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015562 - efflux transmembrane transporter activity
biological_process GO:0006865 - amino acid transport
GO:0070301 - cellular response to hydrogen peroxide
GO:0033228 - cysteine export across plasma membrane
Note(s): Note(s): ...[more].
Reference(s): [1] Dassler T., et al., 2000
[2] Franke I., et al., 2003
[3] Maier TH. 2003
External database links:  
ALPHAFOLD:
 P31125
ECOCYC:
 EG11639-MONOMER
ECOLIWIKI:
 b1533
INTERPRO:
 IPR000620
PFAM:
 PF00892
PRIDE:
 P31125
REFSEQ:
 NP_416050
UNIPROT:
 P31125


Operon      
Name: eamA         
Operon arrangement:
Transcription unit        Promoter
eamA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1841 1621167 reverse nd [RS-EPT-CBR] [4]
  promoter ydeEp4 1621264 forward nd [COMP-AINF] [5]
  promoter TSS_1842 1621290 forward nd [RS-EPT-CBR] [4]
  promoter ydeEp1 1621304 forward nd [COMP-AINF] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Dassler T., Maier T., Winterhalter C., Bock A., 2000, Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway., Mol Microbiol 36(5):1101-12

 [2] Franke I., Resch A., Dassler T., Maier T., Bock A., 2003, YfiK from Escherichia coli promotes export of O-acetylserine and cysteine., J Bacteriol 185(4):1161-6

 [3] Maier TH., 2003, Semisynthetic production of unnatural L-alpha-amino acids by metabolic engineering of the cysteine-biosynthetic pathway., Nat Biotechnol 21(4):422-7

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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