RegulonDB RegulonDB 10.8: Gene Form
   

metI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

metN metQ metI gmhB Fis Fis Fis Fis MetJ MetJ anti-anti-terminator gmhBp gmhBp metNp metNp TSS_415 TSS_415 TSS_414 TSS_414 TSS_413 (cluster) TSS_413 (cluster) TSS_412 TSS_412 TSS_411 (cluster) TSS_411 (cluster) TSS_410 TSS_410 TSS_409 TSS_409 TSS_408 (cluster) TSS_408 (cluster) TSS_407 TSS_407 TSS_406 TSS_406 TSS_405 TSS_405 TSS_404 TSS_404 TSS_403 TSS_403 TSS_402 TSS_402 TSS_401 TSS_401 TSS_400 TSS_400 TSS_399 TSS_399

Gene      
Name: metI    Texpresso search in the literature
Synonym(s): ECK0198, EG11737, b0198, metD, yaeE
Genome position(nucleotides): 220968 <-- 221621 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.29
Reference(s): [1] Merlin C., et al., 2002
External database links:  
ASAP:
 ABE-0000668
ECHOBASE:
 EB1688
OU-MICROARRAY:
 b0198
PortEco:
 metI
STRING:
 511145.b0198
COLOMBOS: metI


Product      
Name: L-methionine/D-methionine ABC transporter membrane subunit
Synonym(s): MetD, MetI, YaeE
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 23.256
Isoelectric point: 10.35
Motif(s):
 
Type Positions Sequence
32 -> 216 LPVGVLLYVTRPGQIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYSAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTR
13 -> 204 VWETLAMTFVSGFFGFVIGLPVGVLLYVTRPGQIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYSAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQ
152 -> 172 ITLITLVGYSAMGGAVGAGGL
186 -> 206 ATVMNTVLVLLVILVYLIQFA
20 -> 40 TFVSGFFGFVIGLPVGVLLYV

 
Classification:
Multifun Terms (GenProtEC)  
  6 - cell structure --> 6.1 - membrane
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.m - ABC superfamily, membrane component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
molecular_function GO:0005515 - protein binding
GO:0048474 - D-methionine transmembrane transporter activity
GO:0015191 - L-methionine transmembrane transporter activity
biological_process GO:0006865 - amino acid transport
GO:0048473 - D-methionine transport
GO:0055085 - transmembrane transport
GO:0015821 - methionine transport
GO:1902475 - L-alpha-amino acid transmembrane transport
Note(s): Note(s): ...[more].
External database links:  
DIP:
 DIP-11198N
ECOCYC:
 METI-MONOMER
ECOLIWIKI:
 b0198
INTERPRO:
 IPR000515
INTERPRO:
 IPR035906
PDB:
 3TUZ
PDB:
 3TUJ
PDB:
 3TUI
PDB:
 3DHW
PDB:
 6CVL
PFAM:
 PF00528
PRIDE:
 P31547
PRODB:
 PRO_000023214
PROSITE:
 PS50928
REFSEQ:
 NP_414740
SMR:
 P31547
UNIPROT:
 P31547


Operon      
Name: metNIQ         
Operon arrangement:
Transcription unit        Promoter
metNIQ


Transcriptional Regulation      
Display Regulation             
Activated by: HypT
Repressed by: MetJ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_399 220386 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_400 220394 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_401 220539 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_402 220543 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_403 220638 forward nd [RS-EPT-CBR] [2]
  promoter TSS_404 220964 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_405 220970 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_406 220975 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_407 221009 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_408 (cluster) 221015 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_409 221017 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_410 221019 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_411 (cluster) 221041 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_412 221053 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_413 (cluster) 221681 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_414 221903 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_415 221928 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Merlin C., Gardiner G., Durand S., Masters M., 2002, The Escherichia coli metD locus encodes an ABC transporter which includes Abc (MetN), YaeE (MetI), and YaeC (MetQ)., J Bacteriol 184(19):5513-7

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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