RegulonDB RegulonDB 11.0: Gene Form
   

chaA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

chaA chaB rdlC TSS_1552 TSS_1552 TSS_1551 TSS_1551 TSS_1550 (cluster) TSS_1550 (cluster) TSS_1549 TSS_1549 ldrCp4 ldrCp4

Gene      
Name: chaA    Texpresso search in the literature
Synonym(s): ECK1210, EG11753, b1216
Genome position(nucleotides): 1270749 <-- 1271849
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 48.77
External database links:  
ASAP:
 ABE-0004082
CGSC:
 30293
ECHOBASE:
 EB1703
ECOLIHUB:
 chaA
OU-MICROARRAY:
 b1216
STRING:
 511145.b1216
COLOMBOS: chaA


Product      
Name: Na+/K+:H+ antiporter ChaA
Synonym(s): ChaA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 39.168
Isoelectric point: 6.649
Motif(s):
 
Type Positions Sequence Comment
17 -> 37 LIFPVLALVVLFLWGSSQTLP UniProt: Helical.
38 -> 58 VVIAINLLALIGILSSAFSVV UniProt: Helical.
40 -> 190 IAINLLALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTLMRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMALPAANFSTGQALLVALISAAMYGVFLLIQT
75 -> 95 LILSLSVVILEVSLISALMAT UniProt: Helical.
85 -> 85 E E → H/D: significant reduction in Na+:H+ exchange (at alkaline pH)

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015297 - antiporter activity
GO:0015385 - sodium:proton antiporter activity
GO:0015386 - potassium:proton antiporter activity
biological_process GO:0010446 - response to alkaline pH
GO:0006811 - ion transport
GO:0055085 - transmembrane transport
GO:0035725 - sodium ion transmembrane transport
GO:0071805 - potassium ion transmembrane transport
GO:0098656 - anion transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Kapitza PL. 1979
[2] Shijuku T., et al., 2002
[3] Trchounian A., et al., 1999
External database links:  
ALPHAFOLD:
 P31801
ECOCYC:
 CHAA-MONOMER
ECOLIWIKI:
 b1216
INTERPRO:
 IPR004713
INTERPRO:
 IPR004837
PFAM:
 PF01699
PRIDE:
 P31801
PRODB:
 PRO_000022269
REFSEQ:
 NP_415734
UNIPROT:
 P31801


Operon      
Name: chaA         
Operon arrangement:
Transcription unit        Promoter
chaA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ldrCp4 1270546 reverse nd [ICWHO] [4]
  promoter TSS_1549 1271993 forward nd [RS-EPT-CBR] [5]
  promoter TSS_1550 (cluster) 1272028 forward nd [RS-EPT-CBR] [5]
  promoter TSS_1551 1272095 forward nd [RS-EPT-CBR] [5]
  promoter TSS_1552 1272231 forward nd [RS-EPT-CBR] [5]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Kapitza PL., 1979, Plasma and the controlled thermonuclear reaction., Science 205(4410):959-64

 [2] Shijuku T., Yamashino T., Ohashi H., Saito H., Kakegawa T., Ohta M., Kobayashi H., 2002, Expression of chaA, a sodium ion extrusion system of Escherichia coli, is regulated by osmolarity and pH., Biochim Biophys Acta 1556(2-3):142-8

 [3] Trchounian A., Kobayashi H., 1999, Fermenting Escherichia coli is able to grow in media of high osmolarity, but is sensitive to the presence of sodium ion., Curr Microbiol 39(2):109-14

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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