RegulonDB RegulonDB 11.1: Gene Form
   

hybC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hybC hybB hybA hybD hybE hybF TSS_3309 (cluster) TSS_3309 (cluster) TSS_3308 TSS_3308 TSS_3307 TSS_3307 TSS_3306 TSS_3306

Gene      
Name: hybC    Texpresso search in the literature
Synonym(s): ECK2988, EG11801, b2994
Genome position(nucleotides): 3141286 <-- 3142989
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.93
External database links:  
ASAP:
 ABE-0009828
CGSC:
 33418
ECHOBASE:
 EB1749
ECOLIHUB:
 hybC
OU-MICROARRAY:
 b2994
STRING:
 511145.b2994
COLOMBOS: hybC


Product      
Name: hydrogenase 2 large subunit
Synonym(s): HybC, hydrogenase 2 alpha; subunit
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 62.491
Isoelectric point: 6.237
Motif(s):
 
Type Positions Sequence Comment
2 -> 552 SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVASGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVH UniProt: Hydrogenase-2 large chain.
39 -> 552 RGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVASGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVH
553 -> 567 VVDADGNEVVSVKVL

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.4 - energy production/transport --> 1.4.1 - electron donors
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0031236 - extrinsic component of periplasmic side of plasma membrane
GO:0044569 - [Ni-Fe] hydrogenase complex
molecular_function GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0016151 - nickel cation binding
GO:0005506 - iron ion binding
GO:0008901 - ferredoxin hydrogenase activity
GO:0033748 - hydrogenase (acceptor) activity
biological_process GO:0009061 - anaerobic respiration
GO:0019645 - anaerobic electron transport chain
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0ACE0
DIP:
 DIP-36022N
ECOCYC:
 HYBC-MONOMER
ECOLIWIKI:
 b2994
INTERPRO:
 IPR001501
INTERPRO:
 IPR018194
INTERPRO:
 IPR029014
PDB:
 6SYO
PDB:
 6GAN
PDB:
 6GAM
PDB:
 6G7M
PDB:
 6EN9
PDB:
 6EHQ
PDB:
 6EHS
PFAM:
 PF00374
PRIDE:
 P0ACE0
PRODB:
 PRO_000022949
PROSITE:
 PS00508
PROSITE:
 PS00507
REFSEQ:
 NP_417468
SMR:
 P0ACE0
UNIPROT:
 P0ACE0


Operon      
Name: hybOABCDEFG         
Operon arrangement:
Transcription unit        Promoter
hybOABCDEFG


Transcriptional Regulation      
Display Regulation             
Repressed by: ArcA, NarL


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3306 3141794 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3307 3144533 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3308 3145033 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3309 (cluster) 3145041 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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